摘要
以高丛越橘"奥尼尔"的带芽茎段为外植体,采用组织培养技术,研究了不同配比的ZT、6-BA、NAA和IBA植物激素对高丛越橘"奥尼尔"外植体诱导的影响。结果表明:最佳的外植体消毒条件为0.1%HgCl_24min,萌芽率可达71.4%。腋芽诱导的初代培养宜用WPM+1.0mg/L ZT培养基,侧枝能够萌发,苗高4.7cm;适宜的增殖培养基为WPM+2.0mg/L ZT,增殖系数达到13.0;试管苗的生根培养基为1/2WPM+0.1mg/L IBA。当试管苗长至5cm出瓶移栽,成活率可达80%以上。
Taking stems with buds of Vaccinium corymbosum ‘O＇Neal＇as explants,the effect of different combination of ZT,6-BA,NAA and IBA added into the media were tested.The results showed that the optimal disinfection treatment was soaking into 0.1% HgCl2 for 4 min with induction rate of 71.4%.The optimal medium for bud induction was WPM＋1.0 mg/L ZT on which some branches germinated and plantlet length reached to be 4.7cm.The suitable proliferation medium was WPM＋2.0 mg/L ZT and the proliferation coefficient achieved to be 13.0.The optimum medium for root induction was 1/2WPM＋0.1mg/L IBA.The plantlets reached to be 5cm in length and could be transplanted outside and the survival rate exceeded 80%.
出处
《北方园艺》
CAS
北大核心
2016年第9期102-105,共4页
Northern Horticulture
基金
广东省科技计划资助项目(2013B020304006)
广东省科技计划资助项目(广东省落叶果树工程技术研究中心)(2015B090903082)
广东省科技厅产学研合作资助项目(2013B090200065)
关键词
高丛越橘“奥尼尔”
组织培养
增殖
Vaccinium corymbosum ‘O＇Neal＇
tissue culture
proliferation
作者简介
李森（1982-），男，硕士，助理研究员，现主要从事园艺植物良种繁育与商品化生产研究及科研管理等工作。E—mail：88045361@qq．com．
责任作者：张施君（1974-），女，博士，副研究员，现主要从事园艺植物育种与栽培研究及教学等工作。E—mail：guanyehuazu@163．com．
