摘要
为快速检测出食用肉类中掺假物质,确保消费者切身利益,维持正常市场秩序,提升贵州省产品质量监督检验的检测能力,采用实时荧光定量PCR对猪源性成分和牛源性成分进行检测。结果表明:荧光PCR可以用来检测猪、牛等动物源性成分,且检测灵敏度为1%;荧光PCR检测动物源性成分提高了检测效率及灵敏度,降低了假阳性机率。
In order to rapidly detect adulterants in meat,ensure consumers＇ vital interests,maintain a normal market order and enhance the detectability of Guizhou Province Product Quality Supervision and Inspection Institute,the bovine derived materials and porcine derives materials were detected by using realtime quantitative PCR.Results：Real-time quantitative PCR was suitable for detecting bovine derived materials and porcine derives materials with detection sensitivity of 1%;Detection efficiency and sensitivity were enhanced and false positive rate was decreased.
出处
《贵州农业科学》
CAS
2016年第4期130-132,共3页
Guizhou Agricultural Sciences
关键词
实时荧光定量PCR
动物源性成分
牛
猪
检测
real-time quantitative PCR
animal-derived materials
bovine
pig
detection
作者简介
左泽彦（J985-），男，工程师，硕士，从事食品检测、动物源性和转基因检测研究。E—mail：zuozeyan2008@163．com
通讯作者：孙端方（1987-），男，高级工程师，博士，从事食品检测研究。E-mail：340109925@qq．com
