摘要
为了建立鸭源性成分的实时荧光检测方法,在Perl语言环境下应用生物信息学分析技术,筛选鸭物种核基因特异性片段并设计特异性引物和Taqman探针。以19个物种的基因组DNA为研究对象,经过特异性验证、体系优化、灵敏度分析和重复性试验,建立了肉制品中鸭源性成分的实时荧光鉴别检测方法。结果表明:检测体系具有较高的特异性和稳定性,灵敏度约为10-3 ng/μL鸭源DNA,能够检测到样品中0.1%鸭肉含量。所建立的方法能准确快速检测肉制品中鸭源性成分,可用于肉制品中鸭源性成分的鉴别检测。
In order to establish a real-time fluorescence detection method for duck-derived components,this paper applied bioinformatics analysis technology in the Perl language environment to screen out specific fragments of duck species nuclear genes,and designed primers and Taqman probes.The experiment used the genomic DNA of 19 species as the research object,and established a real-time fluorescence identification method for duck-derived components in meat products through the process of specific verification,system optimization,sensitivity analysis and repeatability test.The results showed that the detection system has high specificity and stability,and the sensitivity is about 10-3 ng/μL duckderived DNA.The system can detect 0.1%duck meat content in the sample.The established method can be accurately and quickly detect duck-derived components in meat products,and can be used for identification of duck-derived components in meat products.
作者
郝光飞
王磊
陈奕帆
杨园园
赵山山
HAO Guangfei;WANG Lei;CHEN Yifan;YANG Yuanyuan;ZHAO Shanshan(College of Life Science and Food Engineering,Hebei University of Engineering,Handan 056000)
出处
《食品科技》
CAS
北大核心
2019年第12期360-365,共6页
Food Science and Technology
基金
河北省自然科学基金项目(C2016402014)
邯郸市科学技术研究与发展计划项目(1622201049).
关键词
鸭源性成分
实时荧光PCR
肉制品
检测方法
细胞核基因
duck-derived ingredients
real-time fluorescent PCR
meat products
detection method
nuclear gene
作者简介
郝光飞(1982—),男,河北保定人,博士,副教授,研究方向为食品营养与健康。
