摘要
目的:建立天钩降压胶囊内容物正丁醇萃取液的高效液相指纹图谱的检测方法。方法:采用高效液相色谱法,色谱柱TSKgel ODS-100V色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸水溶液,梯度洗脱,柱温30℃,流速1 m L·min-1,检测波长230 nm。结果:建立了天钩降压胶囊内容物正丁醇萃取液指纹图谱共有模式,并分别指认其中的4个色谱峰。通过与对照品的保留时间和紫外光谱图比较,正丁醇萃取液指纹图谱中6,8,9,14号峰的归属分别为:天麻素,松脂醇二葡萄糖苷,黄芩苷,紫丁香苷。10批天麻枣仁安神胶囊正丁醇萃取液的HPLC指纹图谱相似度均大于0.88。结论:该方法灵敏度高、稳定性和准确性良好,为天钩降压胶囊质量标准建立提供了可靠的科学依据。
Objective： To establish the detection method of fingerprint of Tiangou Jiangya Capsules by HPLC-DAD. Methods：The finger print was established by HPLC with Diode Array Detector（ DAD） and TSKgel ODS-100 V column（ 250 mm × 4. 6mm,5 μm）,gradient eluted with acetonitrile-0. 1% phosphoric acid as mobile phase. The flow rate was 1. 0 m L·min-1. The detection wavelength was at 230 nm and column temperature at 25 ℃. Results： HPLC fingerprint of Tiangou Jiangya Capsules were established. A total of 14 common peaks were marked and attributed to the herbs and 4 common peaks determined by the relative time of the standard substance. The similarity of the 10 batches of samples was above 0. 88. Conclusion： The established HPLC-DAD fingerprint is accurate,stable and reproducible,which could reflect the overall characteristics of Tiangou Jiangya Capsules and improve the specificity for quality control of Chinese herbal formula.
出处
《辽宁中医杂志》
CAS
北大核心
2016年第4期804-806,I0003,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家"重大新药创制"科技重大专项(2012ZX09102-201-004)
作者简介
张惠惠（1991-），女，山东威海人，硕士研究生，研究方向：中药新药开发及新剂型研究。
通讯作者：代龙（1964-），男，山东济南人，教授，研究方向：中药新制剂、新技术研究，E-mail：dailongdailong@263．net。
