摘要
本试验通过合成促性腺激素类的抑制激素——抑制素（inhibit hormone,INH）与促性腺激素抑制激素（gonadotropin inhibiting hormone,GnIH）的INH表位多肽疫苗和GnIH表位多肽疫苗,主动免疫甘肃高山细毛羊并对促卵泡素（follicle stimulating hormone,FSH）和促黄体素（luteinizing hormone,LH）激素水平进行比较分析。试验选用15只处在非发情季节的3.5岁的甘肃高山细毛羊,注射INH表位多肽疫苗与GnIH表位多肽疫苗分别为表位多肽疫苗A组和表位多肽疫苗B组,对照组注射等量生理盐水。采血后进行免疫,每隔7d免疫和采血,同时在发情后每隔15min采集一次血液,分离血清,用ELISA试剂盒检测抗体水平及FSH和LH激素变化。结果表明,表位多肽疫苗A组和表位多肽疫苗B组在初次免疫后都产生了相应的抗体,且表位多肽疫苗B组较表位多肽疫苗A组产生的抗体浓度高,75、90、105min时,表位多肽疫苗B组相对表位多肽疫苗A组显著促进了FSH的分泌水平（P〈0.05）。在45～105min时,表位多肽疫苗B组相对表位多肽疫苗A组显著促进了LH的分泌水平（P〈0.05）,且在90到105min时表位多肽疫苗A组和表位多肽疫苗B组血清中FSH和LH的水平均显著高于对照组（P〈0.05）。本试验结果表明,INH表位多肽疫苗和GnIH表位多肽疫苗主动免疫促进了甘肃高山细毛羊的FSH、LH的分泌,GnIH表位多肽疫苗在甘肃高山细毛羊上具有更好的免疫作用,本研究为该表位多肽疫苗在绵羊上的运用奠定了基础。
This study aimed to detect the reproductive hormones levels of follicle-stimulating hormone（FSH）and luteinizing hormone（LH）in Gansu Alpine Fine-wool sheep by active immunization of two synthesized epitope peptide vaccines A and B which were combined inhibit hormone（INH）and gonadotropin inhibit hormone（GnIH）.15 Gansu Alpine Fine-wool sheep were selected which were 3.5year old and lived in anoestrus season,3groups each had 5sheep,GnIH epitope peptide vaccine and INH epitope peptide vaccine were named epitope peptide vaccine A and B groups,while blank group was injected with saline.The blood was collected before immunization and immuned,and bloods were collected once every 7days,meanwhile collected blood every15 min after estrus.The serum was used to detect antibody,FSH and LH levels by ELISA Kits.The results showed that the epitope peptide vaccine A and B groups produced the corresponding antibodies after the initial immunization,and epitope peptide vaccine B group produced more antibodies than epitope peptide vaccine A group.Epitope peptide vaccine B group promoted the FSH secretion levels was significantly higher than epitope peptide vaccine A group in 75,90 and 105min（P〈0.05）;In 45 to 105minutes,the LH secretion levels of epitope peptide vaccine B group was significantly higher than epitope peptide vaccine A group（P〈0.05）,while the LH and FSH levels in epitope peptide vaccine A and B groups were significantly higher than control group（P〈0.05）.This study indicated that the epitope peptide vaccine A and B groups could promote the FSH and LH secretion levels through active immunity in Gansu Alpine Fine-wool sheep and had a good immune function.This study laid a foundation for the application of epitope peptide vaccine on sheep.
出处
《中国畜牧兽医》
CAS
北大核心
2016年第4期1039-1044,共6页
China Animal Husbandry & Veterinary Medicine
基金
甘肃省青年自然基金
非繁殖季节GnIH基因免疫对藏羊卵泡发育的调节机制
国家绒毛用羊产业技术体系
分子育种岗位(NYCY+X-40-3)
中国农业科学院羊育种工程研究中心
关键词
促性腺激素抑制激素
抑制素
主动免疫
甘肃高山细毛羊
生殖激素
gonadotropin inhibit hormone（GnIH）
inhibit hormone（INH）
active immune
Gansu Alpine Fine-wool sheep
reproductive hormones
作者简介
张玲玲（1989-），女，河南开封人，硕士生，研究方向：动物遗传育种与繁殖，E—mail：15890388385@163．com
通信作者：杨博辉，甘肃白银人，研究员，博士生导师，研究方向：动物遗传育种与繁殖，E-mail：yangbh2004@163．com
