摘要
棉籽饼粕是一类重要的蛋白饲料资源,但棉粕中有毒物质游离棉酚的存在影响了其在畜牧业中的使用。通过醋酸棉酚与菌株共培养的方法,筛选获得了一株在液体培养条件下棉酚脱除率达90%以上的菌株ST-141,通过16S r DNA序列比对分析和Biolog微生物鉴定,确定该菌株为枯草芽孢杆菌(Bacillus subtilis)。利用该菌株与酿酒酵母N5对棉粕进行混菌固态发酵,30℃发酵48h后棉粕中游离棉酚脱除率达48.5%,同时粗蛋白含量增加19%,氨基酸总量上升22.2%,必需氨基酸含量增加28.4%,表现出良好的棉籽饼粕固态发酵应用潜力。
Cottonseed meal is an important protein feed resource,however the existence of free gossypol af-fects its application in animal husbandry.In this paper,a strain ST -141,which could remove more than 90% of free gossypol in the liquid fermentation was isolated and identified as Bacillus subtilis through 16S rDNA sequence analysis and Biolog microbial identification.Then the ST -141,mixed with the strain of Saccharomyces cerevisiae N5,were used for the solid state fermentation of cottonseed meal.After incubated at 30 ℃ for 48 h,the removal rate of free gossypol reached 48.5%,and the contents of crude protein,to-tal amino acid and essential amino acid were also increased by 19%,22.2% and 28.4% respectively, showing high application potential.
出处
《粮油食品科技》
2016年第1期81-85,共5页
Science and Technology of Cereals,Oils and Foods
基金
中央级公益性科研院所基本科研业务费专项资金课题(ZX1505)
"十二五"国家科技支撑计划项目(2011BAD26B01-3)
关键词
棉粕
游离棉酚
枯草芽孢杆菌
固态发酵
Cottonseed meal
Free gossypol
Bacillus subtilis
Solid state fermentation
作者简介
王晓玲,1991年出生,女,硕士研究生.
关锋,1978年出生,男,教授.
