摘要
为提高棉粕蛋白资源的利用率,考察了复合蛋白酶水解脱酚棉粕制备多肽的工艺。以多肽得率和水解度为评价指标,比较了不同蛋白酶对棉粕蛋白水解能力的差异,确定了以胰蛋白酶与米曲霉蛋白酶按照酶活比3∶1构建的复合蛋白酶作为水解棉粕蛋白的最佳蛋白酶。采用单因素试验及响应面试验对影响棉粕蛋白水解的因素底物蛋白质量浓度、加酶量、p H、酶解温度以及酶解时间进行了分析,并确定了棉粕蛋白水解的最佳工艺条件为:棉粕蛋白质量浓度25 g/L,加酶量5 000 U/g,p H 8. 91,酶解温度50～55℃,酶解时间5. 92 h。在最佳工艺条件下,水解棉粕蛋白的多肽得率可以达到71. 85%。多肽的氨基酸分析结果表明,酶水解制备的多肽能基本保持原棉粕蛋白的氨基酸组成,其氨基酸种类较齐全,8种必需氨基酸含量丰富,营养价值较高。
To improve the utilization ratio of protein resources from cottonseed meal,the preparation process of polypeptides from the hydrolysis of gossypol removed cottonseed meal by composite protease was investigated.Taking the yield of polypeptides and degree of hydrolysis as the evaluation indexes,the different types of proteases on hydrolysis capability of protein from cottonseed meal were compared,and a composite protease composed with trypsin and Aspergillus oryzae protease in a enzyme activity ratio of 3:1 was determined as the optimal protease for the hydrolysis process.Then,the hydrolysis process conditions,including protein mass concentration of cottonseed meal,enzyme dosage,pH,hydrolysis temperature and hydrolysis time were analyzed and optimized by single factor experiment and response surface methodology.And the optimal process conditions were determined as follows:protein mass concentration of cottonseed meal 25g/L,enzyme dosage 5000U/g,pH 8.91,hydrolysis temperature 50-55℃ and hydrolysis time 5.92h.Under the optimal conditions,the yield of polypeptides was 71.85%.The results of amino acid composition showed that polypeptides could effectively maintain the amino acid composition of cottonseed meal protein,the variety of amino acids was complete,contents of eight kinds of essential amino acids were abundant and the nutritive value was high.
作者
潘进权
郑玉霞
张耀尹
PAN Jinquan;ZHENG Yuxia;ZHANG Yaoyin(School of Life Science and Technology,Lingnan Normal University,Zhanjiang 524048,Guangdong,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2018年第12期59-64,共6页
China Oils and Fats
基金
广东省公益研究与能力建设专项资金项目(2015A010107016
2016A010105024)
国家星火计划项目(2013GA780084)
关键词
复合蛋白酶
棉粕
水解
多肽
工艺优化
composite protease
cottonseed meal
hydrolysis
polypeptides
process optimization
作者简介
潘进权(1978-),男,副教授,博士,研究方向为酶工程(E-mail)panjq@lingnan.edu.cn。
