摘要
为了调查酿酒酵母及Etp A酿酒酵母基因工程菌对大鼠小肠黏膜生长发育状况的影响,120只健康断奶SD大鼠,随机分为空白对照组、酿酒酵母菌组、Etp A酿酒酵母基因工程菌组,分别灌胃生理盐水、酿酒酵母菌液、Etp A酿酒酵母基因工程菌液2 m L/只,持续28 d后连续3 d灌胃大肠杆菌菌液。分别取7、14、21和28 d及灌胃大肠杆菌后大鼠的十二指肠、空肠、回肠制作石蜡切片,HE染色,显微镜下观察,记录各段小肠绒毛的宽度、长度、隐窝深度及绒毛长度/隐窝深度比值(V/C)。结果显示:1)与空白对照组相比,酿酒酵母菌组及Etp A酿酒酵母基因工程菌组的十二指肠绒毛长度、V/C在第21和28天时均显著升高(P<0.05);空肠绒毛长度、V/C在第28天均极显著升高(P<0.01)同时隐窝深度显著下降(P<0.05);回肠V/C在第28天显著升高(P<0.05);各肠段绒毛宽度均无显著差异(P>0.05)。2)灌胃大肠杆菌后,与Etp A酿酒酵母基因工程菌组相比,空白对照组及酿酒酵母菌组的空肠绒毛长度、V/C均极显著下降(P<0.01);回肠V/C均显著下降(P<0.05)。结果表明:酿酒酵母和Etp A酿酒酵母基因工程菌均可以促进肠道黏膜发育,提高消化吸收能力;Etp A酿酒酵母基因工程菌对肠道黏膜的保护作用更显著。
The aim of this study is to investigate the effect of Saccharomyces cerevisiae and Etp A gene- engineering S. cerevisiae on intestinal mucosal structure in rats. Healthy weaning SD rats of 120 were divided randomly into 3 groups： control,S. cerevisiae and Etp A gene-engineering S. cerevisiae groups. The rats from the three groups were respectively gavaged saline,S. cerevisiae culture and Etp A gene- engineering S. cerevisiae culture. After 28 days,the three groups were gavaged enterotoxigenic Escherichia coli. At different time points,the duodenum,jejunum and ileum of each rat were fixed for microscopical examination. The width,length,crypt depth and villus length / crypt depth ratio（ V/C） were recorded. The results showed that： 1） Compared with the control group,the duodenal villus length and V/C of S. cerevisiae group and Etp A gene- engineering S. cerevisiae group at 21 d and 28 d were significantly increased（ P〉0. 05）; at 28 d,the villus length and V / C of jejunum were significantly increased（ P〈0. 01） while the crypt depth was significantly decreased（ P〈0. 05）; at 28 d,the V / C of ileum was significantly increased（ P〈0. 05）; there were no significant difference in the width of each intestine villus（ P〈0. 05）. 2） After gavaged with E. coli,compared with Etp A gene- engineering S. cerevisiae group,the villus length and V / C of jejunum in control group and S.cerevisiae group were extremely significantly decreased（ P〈0. 01）; the V / C of ileum was significantly decreased（ P〈0. 05）. The results indicated that S. cerevisiae and Etp A gene- engineering S. cerevisiae could promote intestinal development and improve digestive function,and the protective effect of Etp A gene- engineering S. cerevisiae for intestinal mucosa was more pronounced.
出处
《畜牧与兽医》
北大核心
2016年第2期1-6,共6页
Animal Husbandry & Veterinary Medicine
关键词
酿酒酵母
EtpA酿酒酵母基因工程菌
小肠黏膜结构
Saccharomyces cerevisiae
EtpA gene-engineering S.cerevisiae
intestinal mucosal structure
作者简介
卢晓冉（1990-），女，硕士研究生
通信作者：王兴龙，内蒙古人，研究员，博士生导师，从事兽医微生物学及免疫学，E-mail：wangxl-2006@163．com
