摘要
目的分析探讨多参数流式细胞术在多发性骨髓瘤（MM）早期诊断中的价值。方法回顾性分析64例MM确诊患者初诊时,流式细胞术、骨髓涂片细胞形态学检查、血清蛋白电泳和免疫固定电泳等实验室检查结果,比较各结果阳性率及在MM早期诊断、分型中的优劣。结果 64例MM患者中62例（96.88%）经流式细胞术检出异常浆细胞（a PC）;56例（87.50%）血清免疫固定电泳呈阳性,其中Ig G型占35.94%,Ig A型占18.75%,轻链型占29.69%。45例细胞形态学检查见骨髓瘤细胞,比例为7.5%~90.0%,相对应的流式细胞术检出的瘤细胞比例为1.17%~72.31%,两者呈正相关（r=0.755,P〈0.01）。4种方法对MM的检出率差异有统计学意义（χ2=20.04,P〈0.01）。结论多参数流式细胞术是MM早期诊断最敏感方法,但4种方法结合将更有利于诊断。
Objective To investigate the value of multiparameter flow cytometry（ MFC） in the early diagnosis of multiple myeloma（ MM）. Methods Retrospectively analyze the detection results of FC,BM smear cytomorphology,serum protein electrophresis（ SPE） and immunofixation electrophoresis（ IFE） of 64 patients with MM confirmed,and to compare and analyze the positive detection ratio and the advantages and limitations of these methods in the early diagnosis and typing of MM. Results Aberrant plasma cells（ a PC） were detected from 62 cases（ 96. 88%） by MFC among 64 patients; 56 cases（ 87. 50%） had positive results by IFE,the frequency of immunoglobulin typing showed： Ig G accounted for 35. 94%,Ig A accounted for 18. 75%,and light- chain- only accounted for 29. 69%; myeloma cells were examined from 45 cases by cell morphology,and the proportion was among 7. 5%- 90. 0%,the correspondent MFC proportion was among 1. 17%- 72. 31%. They showed a positive correlation with each other（ r = 0. 755,P〈 0. 01）. There was statistical significance on the differences in positive detection rates of MM with these four methods（ χ2= 20. 04,P〈 0. 01）. Conclusion MFC is the most sensitive method in the early diagnosis of multiple myeloma,but the combination of these methods will be more conductive to diagnosis.
出处
《中国卫生检验杂志》
CAS
2016年第2期236-239,243,共5页
Chinese Journal of Health Laboratory Technology
关键词
多发性骨髓瘤
流式细胞术
形态学
血清蛋白电泳
免疫固定电泳
Multiple myeloma
Flow cytometry
Cytomorphology
Serum protein electrophresis
Immunofixation electrophoresis
作者简介
唐洁（1981-），女，硕士，主治医师，主要从事临床血液检验诊断方面的研究。
