摘要
目的:通过对14个不同产地金银花药材中指标成分绿原酸、木犀草苷、总酚酸、总黄酮的含量进行测定,找出4个成分之间的相关性,为合理评价金银花药材质量提供参考。方法:采用HPLC和UV法测定金银花中绿原酸、木犀草苷、总酚酸和总黄酮的含量;采用相关程度分析方法对4种成分进行相关性分析。结果:以4种成分为综合考察指标,其中山东临沂产金银花药材中绿原酸、木犀草苷、总酚酸、总黄酮含量较高;通过相关性评价,判定金银花中绿原酸与总酚酸呈高度相关性,木犀草苷与总黄酮呈中度相关,绿原酸与木犀草苷呈中度相关,总酚酸与总黄酮呈中度相关。结论:通过对14个不同产地金银花药材指标成分评价及相关分析,为金银花药材质量的评价及合理利用提供实验数据。
Objective： Through the content determination of chlorogenic acid, mignonette glycosides, total phenolic acid, and total flavonoid in flos lonicerae from 14 different regions, to find out the correlation between the four components, and provide a reference for reasonable assessment of the quality of Loniceroe Japonicae Flos. Method： HPLC and UV method were used to determine the content of chlorogenic acid, mignonette glycosides, total phenolic acid and total flavonoid in Loniceroe Japonicae Flos, and correlation analysis method was used to measure their relationships. Result： With the content of four components as the comprehensive indicators, Loniceroe Japonicae Flos from Linyi in Shandong had the highest content in chlorogenic acid, mignonette glycosides, total phenolic acid, and total flavonoid. Through correlation evaluation, chlorogenic acid and total phenolic acid in Loniceroe Japonicae Flos were highly correlated;mignonette glycosides and total flavonoid were moderately correlated;chlorogenic acid and mignonette glycosides were moderately co-related;total phenolic acid and flavonoids were moderately correlated. Conclusion： The evaluation of index components and related analysis of Loniceroe Japonicae Flos from 14 different origins provide experimental data for quality evaluation and rational utilization of Loniceroe Japonicae Flos.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第5期80-83,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(20132X09507005-004)
关键词
金银花
绿原酸
木犀草苷
总酚酸
总黄酮
Loniceroe Japonicae Flos
chlorogenic acid
mignonette glycosides
total phenolic acids
total flavonoids
作者简介
解世全，实验师，从事药物分析研究，Tel：0411-85890176，E—mail：476610364@qq．com
[通讯作者]孟宪生，教授，博士，从事中药药效物质和作用机制研究，TeL：0411-85890185，E-mail：mxsvvv@126．com
