摘要
建立了一种液相色谱-质谱联用法（LC-MS/MS）快速测定鸡蛋中8种氟喹诺酮类药物残留的方法.样品经磷酸-乙腈提取,冷冻离心,旋转蒸发浓缩,初始流动相溶解,然后用正己烷脱脂,有效地去除杂质,随后进行LC-MS/MS定性定量分析.8种氟喹诺酮类药物含量在2～50μg/kg线性关系良好,相关系数R均大于0.9956;添加量在5,10和20μg/kg时,该方法的加标回收率为65.4% ～119.6%,相对标准偏差均小于7.52%.该方法的检出限为0.0005～0.22μg/kg,定量限为0.02～0.74μg/kg,适用于鸡蛋中8种氟喹诺酮类药物残留的定性和定量检测.
A method was established for the determination of eight fluoroquinolones antibiotics in egg sample by liquid chromatography tandem mass spectrometry.The sample was extracted with phosphoric acid and ace-tonitrile.After separated with high speed frozen centrifugation and concentrated with a rotary evaporator, the residue was dissolved with the mobile phase and then defatted with n-hexane.It was then analyzed with LC-MS.The linear ranges were from 2 to 50 μg/kg with the correlation coefficients above 0.995 6 μg/kg for all the 8 fluoroquinolones.The average recoveries of 8 fluoroquinolones were 65.4% ~119.6% at three spiked level:5, 10, 20μg/kg with the RSD lower than 7.52%.The limit of detection was of 0.000 5μg/kg to 0.22 μg/kg.The limit of quantification was of 0.02μg/kg to 0.74μg/kg.The method could be applied to accurate qualitative and quantitative detection of the eight fluoroquinolones in the eggs.
出处
《浙江师范大学学报（自然科学版）》
CAS
2016年第1期53-59,共7页
Journal of Zhejiang Normal University:Natural Sciences
基金
国家自然科学基金资助项目(KYZKY11132)
金华市科技局项目(2014-3-030)
关键词
液相色谱-质谱联用
鸡蛋
氟喹诺酮
残留
liquid chromatography tandem mass spectrometry
egg
fluoroquinolone
residues
作者简介
杨朝琳（1991-）,女,山东青岛人,硕士研究生.研究方向：地理环境与污染控制.
洪华嫦.E-mail：huachang2002＠163.com
