摘要
目的:观察慢性低灌注状态下大鼠脑白质区域血管生成及血脑屏障破坏状况及其可能联系。方法:96只大鼠随机分为假手术组、缺血1周组、缺血2周组、缺血4周组各24只,缺血组大鼠结扎双侧颈总动脉构建慢性低灌注模型,假手术组不结扎。在相应时间点检测各组大鼠脑白质区域微血管密度、血管内皮细胞生长因子(VEGF)m RNA表达水平及伊文思蓝(EB)静脉注射后脑组织内EB浓度。结果:各缺血组白质区域血管网密度较假手术组高(P<0.01),血管密度在缺血2周组中达高峰。各缺血组VEGF m RNA表达水平均较假手术组增高(P<0.05),缺血2周及缺血4周组的VEGF m RNA表达水平较缺血1周组降低(P<0.05)。各缺血组EB浓度均较假手术组增高(P<0.01),缺血2周及缺血4周组EB浓度较缺血1周组增高(P<0.05)。结论:慢性低灌注状态可诱导VEGF表达、促进血管生成,这种血管生成可能参与脑白质区域血脑屏障破坏机制。
Objective：To explore angiogenesis and blood-brain barrier （BBB） disruption under chronic cerebral hypoperfusion status in white matter areas. Methods： Ninty-six male Wistar rats were divided into groups of sham-operated, ischemia 1 week, ischemia 2 weeks and ischemia 4 weeks （n=24 in each group）. Permanent, bilater- al common carotid artery occlusion was used to induce hypoperfusion in forebrain of the rats, and the sham- operated group was not ligated. Capillary density was used to assess the angiogenic level. Quantitative measurement of Evans Blue was used to assess the BBB function. Reverse transcription polymerase chain reaction （RT-PCR） was used to evaluate the expression of vascular endothelial growth factor （VEGF） mRNA. Results： The vessel density in the white matter of the ischemia groups were increased than that of the sham-ope/ated group （P〈0.01）, and the vessel density of the ischemia 2 weeks group was highest. The VEGF mRNA expression of the ischemia groups was higher than that in the sham-operated group （P〈0.05）, and the VEGF mRNA expression in the ischemia 2, 4 weeks groups were decreased compared with that in the ischemia 1 week group （P〈0.05）. The Evans Blue concentrations of the ischemia groups were increased in comparison with that in the sham-operated group （P〈0.05）, and the Evans Blue concentrations in the ischemia 2, 4 weeks groups were higher than that in the ischemia 1 week group （P〈0.05）. Conclusion： Chronic cerebral hypoperfusion can induce angiogenesis which may contribute to BBB disruption in white matter areas.
出处
《神经损伤与功能重建》
2016年第1期9-11,共3页
Neural Injury and Functional Reconstruction
关键词
脑缺血
血脑屏障
血管生成
白质损害
brain ischemia
blood-brain barrier
angiogenesis
white matter impairment
作者简介
通讯作者林琅plexor@163．com
