摘要
目的应用扫描电镜观察雷丸、阿苯达唑和吡喹酮作用后亚洲带绦虫囊尾蚴的形态学变化。方法将亚洲带绦虫囊尾蚴分别放入20%雷丸、1%阿苯达唑和0.04%吡喹酮培养液中。37℃培养18h,清洗后用戊二醛固定,于扫描电镜下观察结构变化并记录结果。试验以含5%猪胆汁的0.9%NaCl溶液为空白对照。结果空白对照组囊尾蚴头节翻出,顶突闭合,周围有短索状皱壁;吸盘内壁形成纵型邹褶,外缘有环形和结节状邹褶;颈部皱褶规则整齐,微毛有棘样尖端;囊部表面呈珊瑚礁状,表面密布微毛,微毛密集、细长连呈网状。雷丸作用后的囊尾蚴头节伸出囊体,水肿严重,顶突呈火山口状,索状皱壁消失,皮肌僵直坏死;吸盘肿胀破损,基底部变厚,横向皱褶消失;颈部皱褶变宽,裂沟加深,有颗粒状物附着,微毛消失,出现溶蚀状孔;囊部表面溶蚀状,僵直坏死,有许多溶蚀状小孔,微毛脱落。阿苯达唑作用后的囊尾蚴头节伸出囊体,顶突收缩,有环形皱褶,皮肌老化,吸盘无明显变化,吸盘内壁有纵型邹褶,外缘有环形皱褶;颈部裂沟增宽,有残留的微毛结构,出现溶蚀状小孔;囊部表面呈丘壑状略平坦,皮肌外观柔和,有许多溶蚀状小孔,可见微毛基部残留结构。吡喹酮作用后的囊尾蚴头节未能翻出;颈部裂沟增宽,微毛消失,皮层变薄,表皮有薄壁空泡结构,有透明杆状物附着;囊部表面凹凸不平溶蚀状,有许多溶蚀状孔,微毛结构消失,有杆状分泌物附着。结论雷丸、阿苯达唑和吡喹酮对亚洲带绦虫囊尾蚴均有破坏作用,3种药物对囊尾蚴表面形态结构的破坏强度以吡喹酮最强、最快,雷丸次之,阿苯达唑最弱。
Objective To using scanning electron microscopy to observe morphology variation of the cysticercus of Taenia asiaticaafter treatment with Omphalia,albendazole,and praziquantel. Methods The cysticercus of T.asiatica was treated with 20% Omphalia,1% albendazole,or 0.04% praziquantel.Physiological saline containing 5% porcine bile served as the blank control.Cysticerci were cultured at 37℃ for 18 hand then were rinsed with PBS（pH 7.2）,fixed with 2.5% glutaraldehyde,and prepared as specimens for scanning electron microscopy.Specimens were observed under a scanning electron microscope. Results In the blank control group,the cysticercus of T.asiatica had a scolex extending to the bladder,a closed anterior end,and short strands that formed folds around the end.The inner wall of suckers of the scolex had vertical folds while the outer surface had annular and truncated folds.The neck folds were arranged regularly and the microtrichia had tapered ends.The surface of the bladder was ridged.The bladder was densely covered with networks of long microtrichia.When the cysticercus of T.asiatica was treated with Omphalia,the scolex extended to the bladder and was severely swollen.The anterior end resembled a volcano.Strands forming folds on the anterior end disappeared.The vesicular wall of the anterior end was stiff and necrotic.Suckers were swollen and ruptured.Suckers had a thick base.Annular folds on the suckers disappeared.Folds widened and fissures in the neck deepened.Granular attachments and eroded holes appeared in the neck.Microtrichia of the neck disappeared.Erosion was evident in the bladder.The surface of the bladder was stiff and necrotic.Eroded holes appeared in the bladder.Microtrichia of the bladder disappeared completely.When the cysticercus of T.asiatica was treated with albendazole,the scolex extended to the bladder.The anterior end had contracted.Annular folds were observed and the vesicular wall was deteriorating.Suckers exhibited subtle changes.The inner wall of suckers had annular folds and the outer surface had vertical folds.Folds on the neck were wider spaced.There were remnants of microtrichia on the neck.Eroded holes appeared in the neck.The bladder had slight projections and depressions and the vesicular wall appeared to be soft.Eroded holes appeared in the bladder and there were remnants of microtrichia.When the cysticercus of T.asiatica was treated with praziquantel,the scolex did not extend to the bladder.Folds on the neck widened.Microtrichia on the neck disappeared and the tegument was gradually thinning.There were numerous pockets on the outer layer of the tegument.Clear,slender attachments appeared on the neck near the bladder.The surface of the bladder was uneven and erosion was evident.Microtrichia disappeared completely and slender attachments appeared in the bladder. Conclusion Omphalia,albendazole,and praziquantel damage the cysticercus of T.asiatica.Of the 3drugs,praziquantel was the most potent and fastest at disrupting the morphology of the cysticercus,followed by Omphalia,while albendazole was the weakest.
出处
《中国病原生物学杂志》
CSCD
北大核心
2015年第12期1112-1118,1123,共8页
Journal of Pathogen Biology
关键词
亚洲带绦虫
囊尾蚴
雷丸
阿苯达唑
吡喹酮
扫描电镜
Taenia asiatica
cysticercus
Omphalia
albendazole
praziquantel
scanning electron microscope
作者简介
通讯作者（简介） 杜娟（1987-），女，贵州人，硕士研究生，主要从事病原生物学基础研究。E—mail：djdujuanjuan@126．com
