摘要
背景：有研究表明神经干细胞移植治疗神经病理性疼痛有一定效果,但神经干细胞移植数量对神经病理性疼痛的疗效并不确切。目的：观察不同数量神经干细胞鞘内注射对坐骨神经半切断大鼠神经病理性疼痛及脊髓背角、背根神经节胶质源性神经营养因子表达的影响。方法：选取1只孕14-16 d SD大鼠制备神经干细胞悬液,分别以细胞数1×10^-3,1×10^-4,1×10^-5,1×10^-6,1×10^-7经坐骨神经半切断伤模型大鼠鞘内注入30μL,并设置模型组和假手术组作对照。记录术前1 d,术后1,3,7,14,21 d机械痛及热痛阈;术后7,21 d免疫组织化学与RT-PCR检测同侧脊髓背角及背根神经节胶质源性神经营养因子的表达。结果与结论：与假手术组比较,其他各组术后1 d痛阈下降,术后7,14 d最低（P〈0.05）;与模型组比较,术后7 d,随着移植神经干细胞的浓度不断增加,神经干细胞1×10^-4,1×10^-5,1×10^-6,1×10^-7组痛阈逐渐升高,脊髓背角及背根神经节胶质源性神经营养因子表达逐渐上调（P〈0.05）;术后21 d,神经干细胞1×10^-5,1×10^-6,1×10^-7组痛阈均与术前差异无显著性意义,但其胶质源性神经营养因子表达在神经干细胞1×10-5组最高（P〈0.05）。说明鞘内注射神经干细胞能缓解大鼠的神经病理性疼痛,注射剂量以1×10^-5神经干细胞效果最佳。
BACKGROUND： Studies have shown that neural stem cell transplantation has a certain effect on neuropathic pain, but the efficacy of transplanted cell number on neuropathic pain is not exactly understood. OBJECTIVE： To observe the effect of different amount of neural stem cells administered intrathecally on the neuropathic pain and expression of glial-derived neurotrophic factor in the spinal cord dorsal horn and dorsal root ganglion in rats after partial sciatic nerve transaction.METHODS： A Sprague-Dawley rat at 14-16 days of pregnancy was used to prepare neural stem cell suspensions that were injected intrathecally into rat models of partial sciatic nerve transaction at doses of 1×10^-3, 1×10^-4, 1×10^-5, 1×10^-6, 1×10^-7 cells per 30 μL, respectively. Additionally, model group and sham-operated group were set up. Threshold values of mechanical and thermal pain were recorded 1 day before operation, 1, 3, 7, 14,21 days after operation. Expressions of glial-derived neurotrophic factor protein and m RNA in the ipsilateral spinal cord dorsal horn and dorsal root ganglion were detected by immunohistochemistry and RT-PCR, respectively, at 7 and 21 days after partial sciatic nerve transaction. RESULTS AND CONCLUSION： Pain threshold values were decreased in all the groups except the sham-operated group at 1 day after operation, and reached the peak at 7 and 14 days after operation（P〈0.05）. Compared with the model group, the pain threshold values and the expression of glial-derived neurotrophic factor protein and m RNA in the ipsilateral spinal cord dorsal horn and dorsal root ganglion were increased gradually in a dose-dependent manner in the 1×10^-4, 1×10^-5, 1×10^-6, 1×10^-7 groups at 7 days after operation（P〈0.05）. At 21 days post-operation, the pain threshold values showed no differences from the preoperative findings in the 1×10^-5, 1×10^-6, 1×10^-7 groups, but the expression of glial-derived neurotrophic factor was significantly higher in the 1×10-5 group than the other groups（P〈0.05）. Taken together, intrathecal transplantation of neural stem cells at a dose of 1×10^-5 is the most effective in alleviating partial sciatic nerve transaction-induced neuropathic pain in rats.
出处
《中国组织工程研究》
CAS
北大核心
2015年第45期7314-7319,共6页
Chinese Journal of Tissue Engineering Research
基金
河北省医学科学研究重点课题(20110515)~~
作者简介
邓默，女，1979年生，汉族，河北省保定市人，2006年河北医科大学毕业，硕士，主治医师。
通讯作者：赵峰，硕士，主治医师，河北大学附属医院介入科，河北省保定市071000
