摘要
【目的】以不同质量浓度的NAA、IBA对选育的栓皮栎、蒙古栎优良种源当年生实生苗半木质化黄化嫩枝扦插生根的影响,探究何种质量浓度下最有利于其扦插生根,以期选出最合适的质量浓度组合,为今后栓皮栎和蒙古栎的无性选育提供技术支持。【方法】本研究以选育的栓皮栎、蒙古栎优良种源当年生实生苗半木质化黄化嫩枝为材料,采用萘乙酸(NAA)、吲哚丁酸(IBA)2种植物生长调节剂,分别设置6个质量浓度(3 000、2 000、1 500、750、600、400 mg/L),并用清水、未黄化实生苗半木质化嫩枝为对照,调查不同质量浓度NAA、IBA对栓皮栎、蒙古栎黄化嫩枝扦插生根的影响。【结果】黄化插穗在生根率、生根数、根长和根径方面均明显优于未黄化插穗,2种植物生长调节剂对黄化栓皮栎、蒙古栎嫩枝插穗不定根诱导差异显著。IBA分别为2 000、1 500、750、600、400 mg/L时处理黄化或未黄化栓皮栎、蒙古栎嫩枝的生根效果均优于NAA相对应的5个不同质量浓度处理的生根效果。在生长调节剂各个质量浓度条件下,处理的黄化栓皮栎、蒙古栎插穗,其不定根发生率、生根数等生根指标均明显高于未黄化插穗所对应的指标。当质量浓度为1 500 mg/L时,栓皮栎黄化嫩枝生根率比未黄化栓皮栎嫩枝的还要高20.0%,蒙古栎黄化嫩枝生根率比未黄化蒙古栎嫩枝的还要高14.6%。【结论】栓皮栎、蒙古栎优良种源当年生实生苗半木质化的黄化与未黄化嫩枝扦插繁殖的最佳植物生长调节剂处理剂量均为1 500 mg/L的IBA。其不定根发生率、生根数等生根指标均最大,其中黄化栓皮栎插穗生根率达100%,黄化蒙古栎插穗生根率达84.6%。
[Objective]The effects of different concentrations of NAA and IBA on the rooting of one-yearold semi-lignified etiolated shoots of the selected provenances of Quercus variabilis and Quercus mongolica were investigated,aiming to find out what kind of mass concentration is most favorable for the rooting of semi-lignification of the etiolated shoot cuttings of current-year seedlings from fine provenances of Quercus variabilis and Quercus mongolica,and to select the most suitable mass concentration,and provide technical support for the asexual selection of Quercus variabilis and Quercus mongolica.[Method]In this study,semi-lignified etiolated shoots of current-year seedlings from selected provenances of Quercus variabilis and Quercus mongolica were used as materials.Two kinds of growth regulators(Butyric acid(IBA)and naphthylacetic acid(NAA))were used.Setting 6 mass concentrations of 3 000,2 000,1 500,750,600,400 mg/L,respectively,and rinsing,non-etiolated semi-lignified shoots of the seedlings were used as control to investigate the effects of different concentrations of IBA and NAA on rooting of etiolated shoots of Quercus variabilis and Quercus mongolica.[Result]The results showed that the etiolated cuttings were superior to the non-etiolated cuttings in rooting rate,rooting number,root length and root diameter.There were significant differences in adventitious rooting induction between two plant growth regulators for shoot cuttings of Quercus variabilis and Quercus mongolica.When the concentration was 2 000,1 500,750,600,400 mg/L for IBA,the rooting effects of treated etiolated or non-etiolated shoots of Quercus variabilis and Quercus mongolica were better than those of NAA.Under each mass concentration of growth regulators,the rooting indexes such as the adventitious root incidence and the number of roots of etiolated Quercus variabilis and Quercus mongolica were significantly higher than those of the non-etiolated cuttings.Under the condition of 1 500 mg/L,rooting rate of the etiolated Quercus variabilis was 20.0%higher than that of the non-etiolated one,and the rooting rate of etiolated Quercus mongolica was 14.6%higher than that of the non-etiolated one.[Conclusion]1 500 mg/L IBA is the best plant growth regulator treatment for the etiolated and non-etiolated semi-lignified shoots of the current-year seedlings of Quercus variabilis and Quercus mongolica from superior provenance.The rooting rate of adventitious roots and rooting number were the highest,among which the rooting rate of etiolated Quercus variabilis cuttings was 100%,and that of etiolated Quercus mongolica cuttings was 84.6%.
作者
张杰
李健康
段安安
孙宇涵
刘洪山
武素然
李贵芬
李云
Zhang Jie;Li Jiankang;Duan An’an;Sun Yuhan;Liu Hongshan;Wu Suran;Li Guifen;Li Yun(Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China;College of Forestry,Southwest Forestry University,Kunming 650224,Yunnan,China;Hongya Mountain State-Owned Forest Farm Administration Bureau of Hebei Province,Yixian 074200,Hebei,China)
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2019年第7期128-138,共11页
Journal of Beijing Forestry University
基金
中央高校基本科研业务费专项（2016BLPX13）
北京林业大学2019年度一流学科建设项目（2019XKJS0308）
北京林业大学重大科研成果培育项目（2017CGP007）
关键词
栓皮栎
蒙古栎
黄化嫩枝扦插
植物生长调节剂
不定根诱导
Quercus variabilis
Quercus mongolica
etiolated shoot cuttings
plant growth regulator
adventitious rooting induction
作者简介
第一作者:张杰。主要研究方向:林木遗传育种。Email:872404121@qq.com。地址:650224云南省昆明市盘龙区白龙寺300号西南林业大学林学院;责任作者:孙宇涵,副教授。主要研究方向:林木遗传育种和生物技术。Email:syh831008@163.com。地址:同上。李云,教授,博士生导师。主要研究方向:林木遗传育种和生物技术。Email:yunli@bjfu.du.cn。地址:100083北京市海淀区清华东路35号北京林业大学生物科学与技术学院。
