摘要
目的：通过比较黄连煎煮液和葛根芩连汤中小檗碱的煎出率大小，阐明中药复方的科学性。方法采用高效液相色谱法（HPLC）分别测定黄连单方和葛根芩连汤复方中小檗碱的含量，色谱条件为 C18色谱柱，以乙腈：0．05 mol／L 磷酸二氢钾（48：52）（每100 mL 加十二烷基硫酸钠0．4 g，用冰醋酸调节 pH 至4．0）为流动相，检测波长为345 nm，流速为1．0 mL／min，进样量10μL，柱温30℃。结果葛根芩连汤中小檗碱的平均煎出率为0．73％，黄连单独煎煮小檗碱的平均煎出率为0．45％，差异有统计学意义（P ＜0．01）。结论中药复方葛根芩连汤的药效大于各单方简单累加而成的药效，故中药复方葛根芩连汤的组成是科学的。
Objective To compare the solution rate of berberine in Gegen Qinlian decoction and Chinese goldthread decoction,to clarify the scientific nature of the traditional Chinese medicine compound.Methods High performance liquid chromatography（HPLC）was used to detect the content of berberine in Gegen Qinlian decoction and Chinese goldthread decoction.Chromatographic condition was C1 8 chromatographic column,mobile phase was acetonitrile：0.05mol/L of potassium dihydrogen phosphate（48：52）（0.4g sodium dodecyl sulfate was added to per 1 00mL solution,adjusted pH to 4.0 with glacial acetic acid）,detection wavelength was 345nm,the sample size was 1 0μL,flow rate was 1 .0mL/min,column temperature was 30℃.Results The average decoct rate of berberine in Gegen Qinlian decoction was 0.73%,while that was 0.45% in Chinese goldthread decoction,there was significant difference between the two groups（P 〈0.01 ）.Conclusion The efficacy of TCMcompound Gegen Qinlian decoction is greater than the efficacy of the unilateral simple accumulation,so traditional Chinese medicine compound has certain scientific nature.
出处
《中国基层医药》
CAS
2015年第24期3712-3714,J0046,共4页
Chinese Journal of Primary Medicine and Pharmacy
基金
安徽省卫生厅中医药科研课题（2014ZY83）
关键词
小檗碱
黄连属
葛根芩连汤
色谱法
高压液相
Berberine
Coptis
Gegen Qinlian decoction
Chromatography,high pressure liquid
作者简介
通信作者：王玉宝，Email：baowang3020509@sina．com
