摘要
烟草黑胫病（Phytophthora nicotianae ）是四川省泸州烟区最为严重的病害之一,为明确泸州烟草黑胫病生理小种组成及其对甲霜灵和烯酰吗啉的敏感性,从泸州主产烟区采集样品,分离得到24株烟草黑胫病菌株；通过鉴别寄主法结合 TTZ 生化反应,发现24株菌株中,0号生理小种15株,占62.50%,1号生理小种9株,占37.50%。对24株菌株进行甲霜灵和烯酰吗啉的药剂敏感性检测,发现甲霜灵对24株菌株的 EC50范围在0.052～0.513μg/mL之间,敏感菌株和中抗菌株分别占41.67%和58.33%,未发现高抗菌株；烯酰吗啉对24株菌株的 EC50范围在0.110～1.615μg/mL 之间,敏感菌株和中抗菌株分别占37.50%和62.50%,未发现高抗菌株。上述结果表明,泸州烟区烟草黑胫病菌同时存在0号生理小种和1号小种,而且该烟区的黑胫病菌已对甲霜灵和烯酰吗啉产生不同程度的抗性。
Tobacco black shank,caused by Phytophthora nicotianae ,is one of the most destructive diseases in Luzhou,Sichuan.In order to confirm the races of P .nicotianae and their sensitivity to metalaxyl and dimetho-morph,24 strains of P .nicotianae were firstly isolated from samples of the tobacco production areas in Luzhou. All 24 isolates were identified as race 0 and race 1 based on host assay and TTZ color reaction.Fifteen isolates were identified as race 0,and 9 isolates were race 1,which accounted for 62.50% and 37.50% respectively.The sensitivity of these isolates to metalaxyl and dimethomorph were measured subsequently.The EC50 values of metal-axyl to these isolates ranged from 0.052 μg/mL to 0.5 13 μg/mL,with the proportions of sensitive and moderate resistant strains of 41.67% and 58.33%,respectively.EC50 values of dimethomorph to these isolates ranged from 0.110 μg/mL to 1.615 μg/mL and the proportions of the sensitive and the intermediate resistant strain were 37.50% and 62.50%,respectively.No high resistant strain to metalaxyl and dimethomorph was found.These results indicated that both of race 0 and race 1 of P .nicotianae existed in Luzhou and these isolates had developed fungicide resist-ance to metalaxyl and dimethomorph.
出处
《植物保护》
CAS
CSCD
北大核心
2015年第6期178-184,190,共8页
Plant Protection
基金
国家烟草专卖局重点项目(110200902065)
四川省烟草专卖局项目(201101007)
关键词
烟草黑胫病
生理小种
药剂敏感性
甲霜灵
烯酰吗啉
Phytophthora nicotianae
physiological race
fungicides sensitivity
metalaxyl
dimethomorph
作者简介
E-mail：zhangchengsheng@caas．cn
