摘要
背景:近年来,干细胞治疗股骨头早期骨坏死已经成为一种可选的方法,但干细胞质量影响治疗效果。目的:评价激素性股骨头坏死模型大鼠骨髓间充质干细胞的增殖能力以及定向诱导分化能力。方法:20只SD大鼠随机分为对照组和观察组,每组10只,观察组构建激素性股骨头坏死模型,分离培养两组大鼠骨髓间充质干细胞。采用CCK-8法检测第3代骨髓间充质干细胞增殖情况。选择第3代骨髓间充质干细胞进行成骨、成脂定向诱导分化。成骨诱导7,14 d行碱性磷酸酶染色和茜素红染色,成脂诱导21 d行油红O染色。结果与结论:培养第1,3,5天观察组细胞吸光度值均低于对照组,差异均无显著性意义(P>0.05);但第7天时观察组吸光度值显著低于对照组,差异有显著性意义(P<0.05)。观察组碱性磷酸酶活性显著低于对照组(P<0.05)。与对照组比较,观察组的钙结节和脂滴数量较少。以上结果表明激素性股骨头坏死模型大鼠骨髓间充质干细胞的增殖能力和成骨成脂诱导分化能力均减弱。
BACKGROUND： In recent years, stem cell therapy for early osteonecrosis of the femoral head has become an alternative method, but the quality of stem cells is a key to the therapeutic outcomes. OBJECTIVE： To evaluate the proliferative ability and directional differentiation ability of autologous bone marrow mesenchymal stem cells in a rat model of steroid-induced femoral head necrosis. METHODS： Twenty Sprague-Dawley rats were randomly divided into control and observation groups with ten in each group. An animal model of steroid-induced femoral head necrosis was built in the observation group, and then bone marrow mesenchymal stem cells from rats in both two groups were isolated and cultured. Cell counting kit-8 was used to detect proliferation of passage 3 cells. Bone marrow mesenchymal stem cells at passage 3 were selected in the two groups for osteogenic and adipogenic induction. Alkaline phosphatase staining and alizarin red staining were adopted at 7 and 14 days of osteogenic induction, and oil red O staining as performed at 21 days of adipogenic induction. RESULTS AND CONCLUSION： The absorbance values of bone marrow mesenchymal stem cells were lower in the observation group than the control group at 1, 3, 5 days of culture, but there was no significant difference between two groups（P 0.05）. Until the 7th day of culture, the absorbance value and alkaline phosphatase activity in the observation group were significantly lower than that in the control group（P 0.05）. Additionally, there were fewer calcium nodules and lipid droplets in the observation group compared with the control group. These findings suggest that the proliferative ability and directional differentiation ability of autologous bone marrow mesenchymal stem cells from a rat model of steroid-induced femoral head necrosis are both decreased.
出处
《中国组织工程研究》
CAS
北大核心
2015年第41期6579-6583,共5页
Chinese Journal of Tissue Engineering Research
作者简介
杨广杰，男，河南省开封市人，2004年郑州大学医学院毕业，主要从事关节和脊柱方面的研究。
通讯作者：黄荣，主治医师，河南大学第一附属医院，河南省开封市475000
