摘要
目的探讨表皮生长因子受体（EGFR）的磷酸化水平与乳腺癌细胞生物学行为及患者术后生存期的关系。方法采用双染免疫组化检测60例乳腺癌组织中EGFR和磷酸化EGFR（p-EGFR）的表达，分析两者与乳腺癌生物学行为、术后无瘤生存期及总生存期的关系。结果60例乳腺癌标本EGFR阳性率81．67％（49／60），p-EGFR阳性率60．0％（36／60）。EGFR阳性率与分化程度密切相关，而p-EGFR则与分化程度、淋巴结转移、临床分期密切相关，在低分化、有淋巴结转移、进展晚期的乳腺癌中表达明显升高。Kaplan—Meier生存曲线及log—rank分析发现，EGFR与乳腺癌患者术后总生存期（Os）和无瘤生存期（DFS）无关，而p-EGFR表达水平与乳腺癌患者无瘤生存期（DFS）显著相关，p-EGFR高表达组患者DFS比阴性组乳腺癌患者缩短。结论乳腺癌组织EGFR蛋白磷酸化激活是影响乳腺癌患者术后生存期的重要因素。EGFR被激活后的磷酸化水平能更好地反映乳腺癌的生物学行为，并对患者的预后判断有一定帮助。
Objective To explore the relationship between phosphorylated levels of epidermal growth factor receptor （EGFR） and the biological behaviors of breast cancer and the postoperative survival of cancer patients. Methods The levels of total EGFR and phosphorylated EGFR （p -EGFR） were detected in sixty breast cancer tissues by double immunohistochemical staining. Then, their relationship with the biological behaviors of breast cancer, the postoperative disease - free survival （DFS） and overall survival in cancer patients were analyzed. Results These cancer specimens showed a positive rate of 81.67% （49/60） for EGFR and 60.0% （36/60） for p - EGFR. The positive rate of EGFR was related with the differentiation of cancer cells. In contrast, the positive rate of p - EGFR was associated with the differentiation of cancer cells, lymph node metastasis and clinical phases. The amount of p - EGFR was remarkably increased in poorly differentiated, advanced cancer with lymph node metastasis. According to the Kaplan - Meier curve and log - rank analysis, the level of EGFR was not related with DFS and overall survival of cancer patients, while the quantity of p - EGFR was closely associated with DFS of cancer patients. The patients presenting a high level of p - EGFR showed a shorter DFS than those with negative p - EGFR expression. Conclusion The phosphorylation of EGFR is an important factor associated with postoperative survival in breast cancer patients. The level of phosphorylated EGFR can reflect the biological behaviors of breast cancer, which is helpful for assessing the prognosis of patients.
出处
《徐州医学院学报》
CAS
2015年第9期571-576,共6页
Acta Academiae Medicinae Xuzhou
关键词
乳腺癌
信号通路
表皮生长因子受体
磷酸化
预后
breast cancer
signaling pathway
epidermal growth factor receptor
phosphorylation
prognosis
作者简介
通信作者，E—mail：xdhuang3970808@sina．com
