摘要
针对酸液浸提葵仁后所得的多酚粗提液(主要含3-O-咖啡酰奎尼酸、4-O-咖啡酰奎尼酸和5-O-咖啡酰奎尼酸),研究大孔吸附树脂对其分离富集的工艺条件。考察9种大孔吸附树脂的吸附解吸性能,筛选出XDA-1树脂用于分离纯化。结果表明:经超滤初步纯化后,粗提液纯度可由4%提高到15%;将此超滤液进一步进行树脂吸附,其优化后的吸附工艺条件为进料液多酚浓度2.0mg/mL、pH 3.0、上样流速2BV/h、上样量30倍床体积(BV);吸附结束后,先采用10%(V/V)的乙醇进行部分去杂,然后用50%(V/V)的乙醇以4BV/h的流速进行洗脱收集。经树脂分离纯化后所得的多酚,其回收率为82.3%,纯度由15%提高到77%。联合超滤和大孔吸附树脂分离是一种从葵花籽提取液中回收CQA的高效经济方法。
An efficient preparative separation and enrichment process of polyphenols from sunflower kernel extracted by acid solution has been developed in the present study.3-O-caffeoylquinic acid（CQA）,4-O-CQA and 5-O-CQA were identified as the main polyphenols in crude extract.The adsorption capacity and desorption ratio for total polyphenols of nine macroporous resins had been evaluated and XDA-1resin was found to be the most suitable resin for the separation of polyphenols.It was found that ultrafiltration as preliminary treatment could improve the purity of crude extract from 4%to 15%.After that,the extract was submitted to XDA-1resin column.The optimized purification conditions were obtained as following：polyphenol concentration in sample solution of 2.0 mg/mL,sample pH of3.0,flow rate of 2bed volume（BV）/h,sample volume of 30 BV.When the adsorption is done,10%（V/V）ethanol was applied to the column to wipe off the impurity at 4BV/h.Then polyphenols were eluted with 50%（V/V）ethanol at 4BV/h.With the enrichment on XDA-1resin,the purity of polyphenol in the final product was increased from 15% to 77%,and recovery yield of 82.3% was obtained.Therefore,combination of ultrafiltration and macroporous resin separation provided an efficient and cost-effective method for recovery of CQAs from sunflower kernel extract.
出处
《食品与机械》
CSCD
北大核心
2015年第5期210-216,共7页
Food and Machinery
基金
国家"863"计划项目(编号:2013AA102104)
江苏省自然科学基金资助项目(编号:BK2012116)
关键词
多酚
大孔吸附树脂
葵仁
分离
富集
polyphenols
macroporous adsorption resin
sunflower kernel
separation
enrichment
作者简介
徐丹丹（1990一-），女，江南大学在读硕士研究生。E—mail：cecilia900116@163．com
通讯作者：张文斌
