摘要
目的应用响应面分析法优化马兰总黄酮的提取工艺。方法以单因素研究为基础,以总黄酮提取量为响应值,以4因素3水平的响应面分析法优化马兰总黄酮的提取工艺。结果马兰总黄酮的最优提取工艺条件为:乙醇浓度为40%,液料比为16.5∶1,提取时间为2h,提取次数3次,在该条件下,马兰总黄酮提取量预测值为162.538mg,实际验证值为156.867mg,并得出各因素对马兰总黄酮提取量的影响大小依次为乙醇浓度、液料比、提取次数、提取时间。结论响应面分析法可用于优化马兰总黄酮的提取工艺,实验验证值与模型预测值基本相符。
Objective To optimize the extraction technique for total flavonoids in Kalimeris indica （L.） Sch-Bip by response surface analysis. Methods Based on the single-factor experiment and with the yield of total flavonoids as the response, a four-factor, three-level response surface analysis was used to optimize the extraction technique for total flavonoids in Kalimeris indica （L.） Sch-Bip. Results The results indicated that in the optimal extraction technique, raw material was treated with 40% ethanol （16.5 times） three times （2 h each time）. In this condition, the predicted and experimental yields of total flavonoids in Kalimeris indica （L.） Sch-Bip were 162. 538 mg and 156. 867 mg, respectively. The most important influencing factor for the yield of total flavonoids was ethanol concentration, followed by solid-liquid ratio, extraction times, and extraction duration. Conclusion Response surface analysis can be used to optimize the extraction technique for total flavonoids in Kalimeris indica （L.） Sch-Bip. The experimental value almost accords with the predicted value.
出处
《安徽中医药大学学报》
CAS
2015年第5期89-93,共5页
Journal of Anhui University of Chinese Medicine
基金
国家自然科学基金项目(81303220)
安徽省自然科学基金项目(11040606W220)
康缘中医药科技创新基金项目(KYCX 201003)
关键词
马兰
总黄酮
提取工艺
响应面分析法
Kalimeris indica （L.） Sch-Bip
total flavonoids
extraction technique
response surface analysis
作者简介
王诤（1991-），男，硕士研究生
通信作者：王国凯，wanggk7779@163．com
