摘要
为了研究陆川猪HSP27基因的遗传变异情况,试验采用RT-PCR和克隆方法,扩增了HSP27基因的编码区序列,并进行了测序和序列分析及其蛋白质结构预测。结果表明:陆川猪HSP27基因编码区全长624 bp,编码207个氨基酸;与Gen Bank中已发表的猪HSP27基因序列相比,核苷酸有1个位点发生碱基突变,但并未引起相应氨基酸的改变;陆川猪HSP27成熟肽包含α螺旋、β转角、延长线和无规卷曲;陆川猪HSP27基因与野猪的亲缘关系最近。
To study the genetic variation of the heat shock protein 27 （ HSP27 ） gene in Luchuan pig, the coding region sequence of HSP27 gene in Luchuan pig was amplified using RT - PCR and a cloning method, and then the sequencing, sequence analysis and the prediction of protein structure of HSP27 were carried out. The results showed that the full - length of the coding region of the HSF27 gene in Luchuan pig was 624 bp, encoding for a protein of 207 amino acids. Compared with the HSP27 gene sequences pubiished in GenBank, a base mutation was detected at one nucleotide site, but it did not cause the change in the corresponding amino acid. The HSP27 mature peptide in Luchuan pig contained α helix, β comer,extension cords and random coils. The Luchuan pig had the closest relationship with Sus scrofa in the HSP27 gene.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2015年第9期120-123,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
国家现代农业产业技术体系广西生猪创新团队项目(nycytxgxcxtd-03-15)
关键词
陆川猪
HSP27基因
克隆
编码区
序列分析
蛋白质二级结构
结构预测
同源性
Luchuan pig
heat shock protein 27 （HSP27） gene
cloning
coding region
sequence analysis
protein secondary structure
structure prediction
homology
作者简介
黄建芳（1990-），女，硕士研究生，研究方向为动物遗传育种，825440271@qq．com．
通信作者：兰干球（1963-），男，教授，博士，研究方向为动物遗传育种和小型猪疾病模型，ganqiulan@gxu．edu．cn．
