摘要
为了提取和鉴定长牡蛎(Crassostrea gigas)贝壳与外套膜中的黑色物质,选取贝壳与外套膜颜色均为黑色的长牡蛎,将贝壳及外套膜进行粉碎、盐酸水解、水浴加热、乙醚抽脂,从3份贝壳中提取出3份黑色固体物质,从3份外套膜中提取出3份黑色固体物质,将提取的黑色固体溶解于0.01m L/L氢氧化钠水溶液中,在150—500 nm的范围内测定紫外吸收光谱,发现其最大吸收峰在210 nm左右,随着波长增加,其吸光值迅速大幅度下降,与公认的黑色素紫外吸收光谱特征一致,从而首次确定长牡蛎贝壳与外套膜中的黑色物质为黑色素。
To extract and identify the pigment in oyster, we selected Pacific oyster Crassostrea gigas with black shell and mantle for experiment. The oyster shells or mantles were dried after flesh removal, crushed and grounded into powder, and treated in HCl acid for hydrolyzation; and then heated in water-bath and degreased with ether. Finally, black particles were extracted. The extracted black particles were dissolved in 0.01 m L/L Na OH dissolution and ultraviolet absorption spectra were generated by an ultraviolet spectrophotometer. The maximum absorption peak was at 210 nm and the absorption values decreased with the wave increasing within 150—500 nm, which manifested the pigment as melanin. Therefore, the extracted black particles from the shells and mantles of oysters were proved to be melanin for the first time.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2015年第4期909-914,共6页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金资助
31302181号
山东省自然科学基金资助
ZR2013CM026号
烟台市科技发展计划资助
2013ZZ357号
鲁东大学引进人才启动基金资助
LY2013032号
关键词
长牡蛎
贝壳和外套膜
黑色素
提取方法
紫外吸收光谱
Pacific oyster
shell and mantle
melanin
extraction method
ultraviolet absorption spectrum
作者简介
通讯作者：王晓通，副教授，E-mail：wangxiaotong999@163．com.
