摘要
目的：建立柱前衍生化HPLC检测不同基原珍珠粉及贝壳粉中7种主要氨基酸的方法。方法：以异硫氰酸苯酯为柱前衍生化试剂,采用C18色谱柱,流动相A：0.1mol/L醋酸钠溶液（用醋酸调pH值至6.5）,流动相B：乙腈,梯度洗脱,检测波长254nm。结果：氨基酸衍生溶液在24h内保持稳定,7种氨基酸线性相关系数均大于0.9995,加样回收率在93.16%~103.31%之间。结论：本方法分离效果好,精密度和重复性良好,可用于珍珠粉及贝壳粉中7种水解氨基酸含量的测定。
Objective： To establish an analysis method for determination of amino acids in pearl powder and conch powder from different origins by HPLC with pre-column derivation. Methods： Hydrolytic amino acids were extracted by acid hydrolysis method,then derivatized with phenyl isothiocyanate（ PITC）. And the samples were analyzed on a C18 column with gradient elution of 0. 1 mol / L sodium acetate solution（ adjusted to p H 6. 5）（ A） and acetonitrile（ B） at the flow rate of 1. 0 m L / min. The detected wavelength was 254 nm. Results： Amino acids derivative solution remained stable in 24 hours. The response was linear for seven amino acids with a correlation coefficient r 0. 9995. The average recoveries were 93. 16% ~ 103. 31%. Conclusion： The method is sensitive with good accuracy and repeatability,which is useful for the determination of amino acids in pearl powder and conch powder from different origins.
出处
《中药材》
CAS
CSCD
北大核心
2015年第4期693-696,共4页
Journal of Chinese Medicinal Materials
基金
国家十二五"重大新药创制"专项(2014ZX09304307-002)
国家自然科学基金青年项目(81202909)
中国食品药品检定研究院中青年研究发展基金(2014A7)
关键词
珍珠粉
贝壳粉
异硫氰酸苯酯
柱前衍生化
氨基酸
HPLC
Pearl powder
Conch powder
Phenyl isothiocyanate（PITC）
Pre-column derivation
Amino acids
HPLC
作者简介
作者简介：陈佳（1983-），女，硕士，助理研究员，研究方向：中药质量控制；Tel：010-67095432，E-mail：chenjia@nifdc．org．cn。
通讯作者：魏锋，Tel：010-7095432，E-mail：weifeng@nifdc．org．cn。
