摘要
建立柱前在线衍生—高效液相色谱法(HPLC)测定发芽糙米中γ-氨基丁酸(GABA)含量的方法。样品以料液比1:5、超声功率(占总功率)60%、在30℃下超声30 min,提取2次。衍生试剂OPA与样品在线混合10次、衍生2 min,用Agilent Eclipse Plus C18柱分离(流速1 mL/min,检测波长338 nm,柱温40℃)。GABA在5~50 mg/L范围内呈良好线性关系,相关系数大于0.999 0;最低检出限0.33 mg/L,最低定量限1 mg/L,方法添加回收率94.80%~98.40%,RSD为1.82%~2.58%。该方法简单快速、灵敏度高、精确,适合发芽糙米中GABA的检测。
A HPLC method with online pre - column derivatization for determination of γ- amino butyricacid （GABA） in germinated brown rice was established. The germinated brown rice sample was extracted two times by ratio of solid to liquid 1：5 , ultrasonic power account for 60 % of total power, at 30℃ for 30 min. Online pre - column derivation 2 min were reacted by using orthophthalaldehyde （ OPA ） derived re-agents and sample after mixing 10 times. The sample was separated on an Agilent Eclipse Plus C 18 column （ at 1.0 mL /min, 338 n m and 40℃ ）. Good linearity was observed in the range of 5 -50 m g / L with the correlation coefficient 〉 0. 999 0; m i n imum detection limit 0. 33 m g/ L ,minimum quantitative detec-tion limit of 1mg/ L,fortified recovery 94.80% -98.40%,RSD 1.82% -2.58%. The proposed meth-od is simple, rapid, sensitive, accurate and suitable for the detection of GAB A in germinated brown rice.
出处
《粮油食品科技》
2017年第3期48-53,共6页
Science and Technology of Cereals,Oils and Foods
关键词
在线衍生
高效液相色谱法
发芽糙米
Y-氨基丁酸
online derivatization
high performance liquid chromatography
germinated brown rice
γ - aminobutyric acid
作者简介
陈雪,1993年出生,女,硕士研究生.
通讯作者：谭斌,1972年出生,男,博士,研究员.
