摘要
为了探讨母体补充苏氨酸锌对子代生长性能、金属硫蛋白（metallothionein,MT1）m RNA表达的影响,实验以苏氨酸锌为受试物,将90只受孕SD大鼠随机分成5组,并于妊娠期第6~15天进行灌胃补充不同剂量苏氨酸锌。母鼠分娩后将仔鼠进行常规饲养至1月龄,测量仔鼠的体质量、身长和尾长,将各组仔鼠断颈处死后取肝脏、肾脏、脾脏和胸骨等组织进行RNA的提取并测定MT1基因相对表达量。结果表明：与空白对照组相比,苏氨酸锌各剂量组仔鼠体质量有显著增加（P〈0.05）。与溶媒对照组和空白对照组相比较,苏氨酸锌各剂量组仔鼠的MT1 m RNA表达量高,且差异显著（P〈0.05）,并且1 000 mg/kg苏氨酸锌处理组各组织中MT1的基因表达量要高于其他组。本结果表明：妊娠期补充苏氨酸锌能显著提高仔鼠MT1 m RNA表达水平,并能在一定程度上增加仔鼠体质量。
To observe the effect of zinc threoninate（Thr-Zn） orally supplemented to pregnant rats on the growth performance and metallothionein（MT） m RNA expression in their offspring,90 pregnant SD rats were randomly divided into 5 groups which were administered daily with Thr-Zn at different doses by gavage during days 6 to 15 of pregnancy.After birthing,the newborn rats were routinely fed for a full month.After measuring the body weight,body length and tail length,the liver,kidney,spleen,and sternum of the offspring rats from each group were harvested for extracting RNA and determining the relative expression levels of MT1 gene.Compared with the blank control group,body weights of the offspring of rats treated with Thr-Zn increased significantly（P 0.05）.Compared with the blank control and solvent control groups,MT1 m RNA expression levels in the offspring of rats treated with Thr-Zn were significantly higher（P 0.05）.The expression level of MT1 m RNA in offspring rats with maternal Thr-Zn treatment at a dose of 1 000 mg/kg was higher than that in other groups.Therefore,maternal Thr-Zn supplementation can significantly improve the expression level of MT1 m RNA and increase the body weights of offspring rats.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第13期235-238,共4页
Food Science
基金
江西省自然科学基金项目(20132BAB204002)
江西省重大科技创新研究项目(20124ACF00400)
江西省教育厅2011年度产学研合作项目(GJJ11002)
江西省科技支撑计划项目(20151BBF60040)
关键词
苏氨酸锌
金属硫蛋白m
RNA
相对表达量
zinc threoninate
metallothionein m RNA
relative expression level
作者简介
王光然（1988-），女，硕士研究生，研究方向为营养保健与功能食品。E-mail：guangr323@163．com
通信作者：谢明勇（1957-），男，教授，博士，研究方向为食品营养学、食品安全、功能保健食品。E-mail：myxie@ncu．edu．cn
