摘要
目的：对2013年4月~2015年5月间采集的103份福建畲族无关个体样本进行Y染色体上11个短串联重复序列（STR）基因座遗传多态性分析。方法：应用Investigator Argus Y-12扩增系统进行复合扩增,用310遗传分析仪进行基因扫描和基因分型。结果：共检出82个等位基因,基因频率范围为0.0097~0.6990,基因多样性GD值为0.4533~0.9596,11个Y-STR基因座共同构成单倍型91种,其中81种单倍型出现1次,8种出现2次,2种出现3次,单倍型多样性HD为0.9728。结论：11个Y-STR基因座在福建畲族具有较高的遗传多态性,对研究群体遗传学和法医学应用有重要意义。
Objective：To explore the genetic polymorphism of 11Y-chromosome short tandem repeats（STR）loci in Fujian She ethnic population.Methods：Investigator Argus Y-12 reagent kit was used to conduct the co-amplification of gene and a genetic analyzer was used for genescan and analysis of the genotype from 103 unrelated individuals.Results：The frequencies range of 82 STR loci detected were 0.0097-0.6990.The gene diversity values（GD）was 0.4533-0.9596.Ninety-one haplotypes were found in 11Y-STR loci,of which 81 were unique,8were found in 2individuals,2in 3individuals,and the haplotypes diversity values（HD）was 0.9728.Conclusion：The genetic distribution of the 11Y-STR loci in Fujian She ethnic population groups shows favorable polymorphisms,which would be very powerful for population genetics and forensic practice.
出处
《中国计划生育学杂志》
2015年第7期451-454,共4页
Chinese Journal of Family Planning
基金
福建省属公益类科研院所基本科研业务专项(2012R110102004)
关键词
福建畲族
Y染色体
短串联重复序列
遗传多态性
Fujian She ethnic population
Y chromosome
Short tandem repeat
Genetic polymorphism
