摘要
目的对飞龙掌血的镇痛作用及相关机制进行实验探讨,为进一步研究开发镇痛中药提供参考。方法采用醋酸扭体法和热板法考察飞龙掌血的提取物对于化学刺激和热刺激引起疼痛的镇痛效果;用酶联免疫法(ELISA法)检测小鼠血清中β内啡肽(β-EP)、前列腺素E2(PGE2)、五羟色胺(5-HT)、一氧化氮(NO)的含量;用Western blotting法检测小鼠脑组织中β-EP、PGE2和阿片μ受体的蛋白表达。结果飞龙掌血提取物能明显减少冰醋酸所致小鼠的扭体次数,且醇提物低于水提物(P<0.01);飞龙掌血提取物能提高小鼠热刺激痛阈值,醇提物与水提物之间无显著性差异(P>0.05);飞龙掌血提取物能增加小鼠血清中的β-EP的含量、降低PGE2及NO的含量,且醇提物更明显(P<0.05),而5-HT的含量无变化(P>0.05);飞龙掌血的提取物还能上调小鼠脑组织中β-EP的蛋白,下调PGE2的的蛋白表达(P<0.05)。结论飞龙掌血具有镇痛作用,且醇提物效果优于水提物,高剂量组优于中、低剂量组,其镇痛机制可能是与通过增加血清中β-EP,降低PGE2、NO的含量,上调脑组织中β-EP的蛋白表达、下调PGE2的表达有关。
Objective To study the analgesic effect of Toddalia Asiatica,and to provide reference for the research and development of analgesic traditional Chinese medicine. Methods Analgesic effect of Toddalia Asiatica＇s extract was investigated by methods of acetic acid writhing and hot plate,and the pain was induced by chemical and heat stimulation. ELISA kit was used to detect the regulation of beta-endorphin（ β-EP）,5-hydroxytryptamine（ 5-HT）,prostaglandin2（ PGE2）,nitric oxide（ NO） in the serum of the rats; western blot was used to detect β-EP,PGE2 and Opioid receptors μ in brain tissue of rats. Results The number of rats writhing induced by acetic acid was decreased by the Toddalia Asiatica＇s extract,and the ethanol extract was less than the water extract（ P 0. 01）. The pain threshold was remarkably improved by the Toddalia Asiatica ＇s extract,and there was no significantly difference between ethanol extract and water extract（ P 0. 05）. The extract could increase the content of β-EP as well as reduce the content of PGE2 and NO in the serum,and the ethanol extract has more remarkably effect（ P 0. 05）; The extract also can increase the content of β-EP as well as reduce the expression of PGE2 in brain tissue（ P 0. 05）. Conclusion Toddalia Asiatica has analgesic effect,and ethanol extract was better than water extract,high dose group was better than low dose group. The analgesic mechanism maybe has the relationship with increasing β-EP,decreasing the content of PGE2 and NO,and the up-regulation of the expression of β-EP and the down-regulation of the expression of PGE2.
出处
《上海中医药杂志》
2015年第7期82-86,共5页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市教委科研创新项目(11YS77)
上海市教委博士点基金项目(K120309)
关键词
飞龙掌血
镇痛
镇痛机制
Toddalia Asiatica Lam
analgesic effect
analgesic mechanism
作者简介
陆怡，女，硕士生，主要从事中药及复方的药效学研究工作
朱国福，教授，博士生导师；E-mail：gfz1998@163．com
[通讯作者]潘颖宜，副教授，硕士生导师；E—mail：pyyl6@126．com。
