摘要
目的:通过液相色谱-四级杆串联飞行时间质谱(LC-Q-TOF),对飞龙掌血根皮的正丁醇极性部位的主要化学成分进行定性分析研究。方法:采用UPLC C18色谱柱,以乙腈-0.1%甲酸水溶液为流动相梯度洗脱;流速0.4mL/min;柱温30℃。质谱分析采用电喷雾(ESI)离子源,数据依赖采集(DDA)、正负离子模式下分别采集数据。针对保留时间、测得的精确相对分子质量,应用MassHunter软件、天然产物数据库,分析比较各色谱峰的一级二级质谱裂解碎片。结果:从飞龙掌血根皮极性部位共鉴定了39个天然化合物,其主要化学成分包括香豆素类、生物碱类、酚羟基酰胺类等。结论:该方法准确、可靠,适用于飞龙掌血药材中化学成分的鉴定研究,为飞龙掌血中的药效物质基础、资源开发利用提供参考。
Objective:To qualitatively analyze the main chemical constituents of polar extract of toddalia asiatica root bark by LC-Q-TOF.Methods:An UPLC C18 column was applied with gradient elution of acetonitrile-0.1%formic acid solution,at a flow rate of 0.4 mL/min and 30℃of column temperature.MS analysis was based on ESI ion source,data dependent acquisition(DDA),positive and negative ions were respectively collected.Through retention times,measured accurate relative molecular mass,one-level and two-level mass fragments of every chromatographic peak were compared by MassHunter software and natural product databases.Results:There were 39 natural compounds identified from polar extract of toddalia asiatica root bark,and the main chemical constituents included coumarins,alkaloids,phenolic amides,et al.Conclusion:The method is accurate,reliable,suitable for identification of chemical constituents in toddalia asiatica,and it provides a reference for material basis,development and utilization of resource of toddalia asiatica.
作者
周威
曾庆芳
罗才荣
宛蕾
张晓燕
ZHOU Wei;ZENG Qing-fang;LUO Cai-rong;WAN Lei;ZHANG Xiao-yan(Guizhou Medical University,Guiyang 550025,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2019年第12期5914-5919,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81360681)
贵州省高层次创新型人才“千层次”项目(No.2016-2018).
作者简介
通讯作者:张晓燕,贵州省贵阳市贵安新区大学城贵州医科大学基础医学院药理学教研室,邮编:550025,电话:0851-88174012,E-mail:drxyzhang@126.com
