摘要
选用工业大麻新品种龙麻一号的下胚轴作为外植体进行组织培养,对无菌苗的获得、植物生长调节剂的选择及浓度等影响工业大麻组织培养的关键因素进行研究,愈伤组织诱导的最适植物生长调节剂组合为1.0mg/L 6-BA和0.5mg/L NAA,不定芽分化的最适激素组合为1.0mg/L KT和0.5mg/L NAA,以1/2MS+0.05mg/L IBA+0.05mg/L NAA激素组合生根效果较好。初步建立工业大麻高效再生体系,愈伤组织诱导率达到91.67%,不定芽分化率达到76.67%,生根率达到75%。
The Hypocotyls of a new industrial hemp cuhivar Long - ma No. 1 were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture such as the physiological state of aseptic seedlings, the selection and concentration of plant growth regulators and so on. The best combination of plant growth regulators was 1.0 mg/L 6 - BA and 0. 5 mg/L NAA for the induetion of callus. The best combination of hormones was 1.0 mg/L KT and 0. 5 mg/L NAA for the differentiation rate of adventitious bud. The best combination was 1/2 MS ＋ 0. 05 mg/L IBA ＋ 0. 05 mg/L NAA for rooting. The efficient regeneration system for industrial hemp was built preliminarily. The induction rate of callus was 91.67%, the differentiation rate of adventitious bud was 76. 67%, the induction rate of root was 75 %.
出处
《中国麻业科学》
2015年第3期126-129,147,共5页
Plant Fiber Sciences in China
基金
黑龙江省科学院青年创新基金资助项目
关键词
工业大麻
组织培养
再生体系
industrial hemp （Cannabis sativa L ）
tissue culture
regeneration system
作者简介
姜颖（1986-），女，吉林通化人，助理研究员：研究方向作物遗传育种。E-mail：bazhujiangying@126．com。
