摘要
目的探讨联合检测自身抗体、免疫球蛋白和补体在系统性红斑狼疮(SLE)诊断和病情判断中的应用价值。方法选取SLE患者54例、其他自身免疫性疾病患者32例和正常对照30例,采用间接免疫荧光法测定抗核抗体(ANA)、免疫印迹法测定抗核提取物抗体(抗ENA抗体)、散射免疫比浊法测定免疫球蛋白和补体C3、C4。结果 SLE患者的ANA、抗ds DNA、抗Sm、抗核小体、抗U1-nRNP、抗核糖体P蛋白、抗组蛋白抗体的检测阳性率分别为87.04%、59.26%、27.78%、29.63%,37.04%、12.96%、27.78%;SLE活动组中抗ds DNA和抗核小体抗体的阳性率高于SLE非活动组,差异具有统计学意义(P<0.05);SLE活动组Ig G、Ig A、Ig M水平高于正常对照组,C3、C4水平低于正常对照组,差异具有统计学意义(P<0.01)。结论自身抗体联合免疫球蛋白和补体检测对SLE患者的临床诊断和病情判断有良好的参考价值。
Objective To evaluate the clinical significances of combined detections of autoantibodies, immunoglobulin and complement in patients with systemic lupus erythematosus. Methods 54 SLE patients, 32 other autoimmune diseases patients and 30 healthy people were involved in the study. ANA was tested by indirect immunofluorescent method （IIF）, ENA was tested by Western blot, the level of IgG, IgA, IgM, C3 and CA were tested by turbidimetric method. Results The positive rates of ANA, anti- dsDNA, anti- Sm, AnuA, anti- U1- nRNP, ARPA and anti- Histone in SLE patients were 87.04%, 59.26%, 27.78%, 29.63%, 37.04%, 12.96% and 27.78% respectively. The positive rates of anti-dsDNA and AnuA in the SLE active group were significantly higher than the SLE inactive group （P 〈 0.05 ）. Immunoglobulin in the SLE active group was significantly higher than the normal control group. The C3 and C4 level was significantly lower than the normal control group （P 〈 0.01 ）. Conclusion The combined detection of autoantibodies, immunoglobulin and complement have great reference value for the clinical diagnosis and illness evaluation of SLE patients.
出处
《标记免疫分析与临床》
CAS
2015年第6期503-505,共3页
Labeled Immunoassays and Clinical Medicine
基金
上海市崇明县科学技术发展资金项目(项目编号:CKY2013-21)
关键词
系统性红斑狼疮
自身抗体
免疫球蛋白
补体
Systemic lupus erythematosus
Autoantibody
Immunoglobulin
Complement
作者简介
周晔（1973-），女，主管技师，本科，主要从事临床免疫方面的工作。Tel：021—69695590；E—mail：colddog19852000@163．com
通讯作者：张莉（1965-），女，主任技师，本科，主要从事临床免疫方面的工作。Tel：021—69695594；E—mail：cmjyk5590@126．com
