摘要
目的观察咳喘停贴剂穴位贴敷对慢性哮喘大鼠Th1下游抑炎细胞因子含量的影响。方法将48只Wistar大鼠随机分成6组:正常组、模型组、布地奈德组、穴位假贴敷组、地塞米松组、穴位贴敷组,每组8只。用卵清蛋白致敏和激发的方法复制慢性过敏性哮喘大鼠模型。模型复制后各组给予相应的治疗,治疗14d后观察各组大鼠支气管肺泡灌洗液(bronchial alveolar lavage fluid,BALF)中干扰素-γ(interferon-γ,IFN-γ)、白细胞介素-2(interleukin-2,IL-2)和IL-12含量。结果与正常组比较,模型组大鼠BALF中IFN-γ、IL-2、IL-12含量均明显降低(P<0.05)。与模型组比较,穴位假贴敷组BALF中IFN-γ、IL-2、IL-12含量无明显变化(P>0.05);布地奈德组、地塞米松组、穴位贴敷组IFN-γ、IL-12含量明显升高(P<0.05),IL-2含量呈升高趋势(P>0.05),但3组之间IFN-γ、IL-2、IL-12含量比较,差异无统计学意义(P>0.05)。结论咳喘停穴位贴敷治疗慢性哮喘大鼠的机制与调节呼吸道中抑炎细胞因子IFN-γ、IL-2、IL-12的分泌异常有关。
Objective To investigate the effects of acupoint sticking therapy with Kechuanting Paste on the content of downstream anti-inflammatory cytokines in T helper 1 cells of rats with chronic asthma. Methods Forty-eight Wistar rats were randomly and equally divided into normal group, model group, budesonide group, sham-acupoint sticking group, dexamethasone group, and acupoint sticking group. A rat model of chronic allergic asthma was established by sensitization and challenging with ovalbumin. The model rats in each group were given specific treatment. The content of interferon-y （IFN-y）, interleukin- 2 （IL-2）, and IL-12 in bronchial alveolar lavage fluid （BALF） of rats in each group was measured after 14 days of treatment. Results The content of IFN-y, IL-2, and IL-12 in BALF of rats in the model group was all significantly lower than that in the normal group （P〈0.05）. There were no significant differences in the content of IFN-y, IL-2, and IL-12 in BALF between the model group and the sham-acupoint sticking group （P〉0.05）. Compared with the model group, the budesonide group, dexamethasone group, and acupoint sticking group had significantly higher content of IFN-y and IL-12 （P〈0.05） and nonsignificant- ly higher content of IL-2 （P〉0.05）, while there were no significant differences in the content of IFN-y, IL-2, and IL-12 between the three groups （P〉0.05）. apy with Kechuanting Paste in the treatment of rats with tion abnormality of anti-inflammatory cytokines including Conclusion The mechanism of acupoint sticking ther- chronic asthma is associated with the regulation of secre- IFN-7, IL-2, and IL-12 in the airway.
出处
《安徽中医药大学学报》
CAS
2015年第3期51-56,共6页
Journal of Anhui University of Chinese Medicine
基金
国家自然科学基金项目(81102632)
江苏省人事厅"六大人才高峰"项目(2014-WSN-015)
关键词
慢性哮喘
咳喘停贴剂
穴位贴敷
抑炎细胞因子
chronic asthma
Kechuanting Pastel
acupoint sticking
anti-inflammatory cytokine
作者简介
刘兰英（1974-），女，副主任医师，硕士研究生导师
