摘要
为确定四川某猪场导致猪细菌性感染死亡的病原,本研究从送检病死猪肺脏样品中分离得到一株细菌,对该菌株进行培养特性观察、生化试验、动物致病性试验、药敏试验、16S r RNA基因的扩增及测序鉴定。结果显示其16S r RNA基因序列与肺炎克雷伯菌(Kpn)序列同源性达99%,表明该菌株为Kpn肺炎亚种;致病性试验结果显示其对小鼠具有强致病性;并且在血平板上出现α溶血现象;药敏试验结果显示分离菌株表现出多重耐药性,毒力较强,并且在培养特性上发生一定的改变。本实验为进一步研究Kpn耐药机制和致病机理奠定了基础。
To determine the pathogenic bacteriain the effected pigs, a bacteria was isolated from the lung of diseased pig and identified as gAebsiella pneumoniaeby biochemical tests, pathogenicity test, and bacteria 16S rRNA gene sequencing, which was 99% homology with K.pneumoniae. In addition, bacterial colonies were formed on blood agar, presenting sight a hemolytic phenomenon. Results of drug sensitivity tests show that the bacterial isolate was only sensitive to gentamicin, amikacin and ceftizoxime; While, resistant to cefoperazone, piperacillin, amoxicillin＋clavulanicacid, tetracycline and sulfamethoxazole etc in the 19 tested drugs. In conclusion, the K.pneumoniae isolated from swine lungs had not only altered in cultural properties, but also has a strong virulence and a serious multi-drug resistance. The isolate would faciliate further the resistance mechanisms and pathogenesis-related research.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2015年第5期375-378,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
"十二五"国家科技支撑计划(2013BAD12B04)
关键词
肺炎克雷伯菌
分离
鉴定
α溶血
Klebsiellapneumoniae
isolation
identification
ct hemolytic
作者简介
林星宇（1990-），男，四川自贡人，硕士研究生，主要从事动物传染病病原分子生物学研究．
通信作者：E-mail：yaanwangyin@tom．com
