摘要
目前水产下脚料不能得到合理利用,被大量废弃。本研究筛选几株能高效发酵水产下脚料的菌株,分解下脚料产生游离态氮、磷、钾等植物所需营养素。从市售微生物肥料、土壤、腐败鱼体中的微生物分离出44株菌,分别进行单一发酵水产下脚料实验、解磷解钾固氮实验、协同拮抗实验以确定能较好共同发酵水产下脚料的菌株;并通过建立聚类树,结合菌株形态学、生理生化特点,推断菌株的生物属性。最终筛选得到的4株菌分别为,GP2食酸菌、GS4假单胞菌、ZP1黑曲霉和ZP3酵母菌。筛选确定的4株菌,具有较好的发酵水产下脚料产生氮、磷、钾等植物所需营养素的能力,且4株菌作用的发酵液中氮、磷、有机质含量均超过国家微生物肥料标准,这4株菌可以用于水产下脚料发酵。
Screening a few microbes that can ferment by-product of fish processing into useful nutrients forplant. We selected 44 microbes from fertilizer of microbes, soil and corrupted fish, then we chose microbes bysome experiments, such as, single fermented experiment , the experiment of nitrogen fixation, insoluble-potassium solubilization and insoluble-phosphate solubilization simultaneously and collaborative antagonismexperiment. Then microbes were identified by morphological observation, physiological and biochemicalexperiments and building the cluster trees based on 16S rDNA or 18S rDNA sequence data of the strains .Finally, GP2 is Acidovorax, GS4 is Pseudomonas sp. , ZP1 is Aspergillus niger, ZP3 is Saccharomyces. GP2,GS4, ZP1, ZP3 can ferment by-product of fish processing into useful nutrients for plant, and they can be usedto ferment by-product of fish processing.
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2015年第3期465-471,共7页
Journal of Shanghai Ocean University
基金
海洋公益性行业科研专项(201305016)
关键词
下脚料
食酸菌
假单胞菌
黑曲霉
酵母菌
鉴定
发酵
by-product of fish processing
Acidovorax
Pseudomonas sp.
Aspergillus niger
Saccharomyces
identification
fermentation
作者简介
赵玉谨（1990-），女，硕士研究生，研究方向为水产品加工与贮藏。E-mail：1006155498@qq．com
通信作者：罗红宇，E．mail：lisa8919@163．COm
