摘要
目的分析徐州地区慢性乙型肝炎(CHB)病人乙型肝炎病毒(HBV)基因型及耐药变异位点的分布特点。方法收集2013年5月—2013年11月在我院就诊的门诊及住院CHB病人216例,采用核酸扩增技术结合荧光标记探针杂交方法检测病人血清HBV DNA,通过测序分析检测病人血清中的HBV基因分型及耐药变异位点。结果本组216例病人中,HBV基因C型207例(95.83%),B型9例(4.17%);病毒变异的85例,无变异的131例,变异率为39.35%;变异位点有rtV173、rtL180、rtA181、rtT184、rtS202、rtM204、rtV207、rtS213、rtV214、rtQ215、rtN236、rtN238。各突变位点中,rtM204和rtL180位点的突变检出率较高,分别为21.30%和13.89%。与拉米夫定、阿德福韦酯、替比夫定耐药相关的病毒基因位点的突变检出率分别为29.63%、20.37%、12.96%。结论徐州地区HBV基因型分布以C型多见,突变株占检测病毒株的39.35%;与拉米夫定和阿德福韦酯耐药相关的基因位点突变检出率相对较高,与恩替卡韦耐药相关的基因位点突变检出率相对较低。
Objective To analyze the gene mutation site and genotyping distribution of hepatitis B virus（HBV）in patients with chronic hepatitis B（CHB）in Xuzhou area. Methods This study consisted of 216 patients with CHB treated in our hospital from May 2013 to November 2013.Employing combined Nucleic Acid Amplification Techniques with fluorescent labeling hybridization method,their serum HBV DNA was detected and HBV genotyping and resistance variation site were analyzed. Results Among 216 patients,207cases（95.83%）were genotype C,nine（4.17%）were genotype B;virus variant was noted in 85 cases,and 131 cases with no variant,aberration rate being 39.35%;variation sites were rtV173,rtL180,rtA181,rtT184,rtS202,rtM204,rtV207,rtS213,rtV214,rtN236 and rtN238.In the mutable points,the detection rates in rtM204 and rtL180were 21.30% and 13.89%,respectively.The mutation rates of lamivudin-,adefovir-,telbivudin-related virogene locus were29.63%,20.37%,12.96%,respectively. Conclusion In Xuzhou area,genotype C is more commonly seen in HBV genotype distribution,the mutation strains accounts for 39.35%in the strains detected.The detection rates are relatively higher in lamivudine-and adefovir-resistance points,but lower in entecavir-related point.
出处
《齐鲁医学杂志》
2015年第2期170-172,共3页
Medical Journal of Qilu
基金
江苏省高校自然科学基础研究面上项目(08KJD-320012)
徐州医学院研究生科技创新计划项目(XYLC-1208)
作者简介
赵玉宝（1988-），男，硕士研究生。
[通讯作者]丁芹（1970-），女，博士，副主任医师，副教授。Email：kgylan163@163．com。
