摘要
研究徐州地区乙型肝炎病毒的基因型分布及其与肝功能损伤、病毒复制水平的关系。采用PCR微板杂交-ELISA技术进行HBVDNA基因分型,并结合其HBVDNA定量值和丙氨酸转氨酶结果,分析乙肝病毒基因型的临床意义。徐州地区乙肝病毒基因型B、C和D型分别占26.7%、44.0%和17.3%,在急性乙型肝炎、慢性乙型肝炎和肝硬化中C基因型的HBVDNA定量值显著高于B型者(t=2.45,2.53和2.36,P=0.027,0.018和0.029)。D型和与D型相关的基因型发生重型肝炎的百分率为51.7%。C基因型是徐州地区的优势基因型,并与较重的肝损伤有关,而B型与较轻的肝损伤有关,D型可能与重型肝炎有关。
To study the distribution of hepatitis B virus genotype in Xuzhou and clarify the genotype-related diffrence in the liver function and level of HBV DNA.HBV genotype was determined by PCR-Microplate sandwich hybridization-ELISA technique.Analysed the clinical significance combining with the value of HBV DNA and ALT.Among them genotype B was 26.7%,genotype C was 44.0%,genotype D was 17.3%.The HBV DNA value of genotype C was significantly higher than that of genotype B in AH,CHB,and HLC,(t=2.45,2.53 and 2.36,P=0.027,0.018 and 0.029),genotype D and genotype which related to geonotybe D is 57.1% in fulminiant hepatic failure(FHF),genotype C is the major genotype in Xuzhou and associated with the development of severe liver disease contrarying with genotype B,genotype D perhaps related with fulminiant hepatic failure.
出处
《临床肝胆病杂志》
CAS
2005年第3期151-152,共2页
Journal of Clinical Hepatology
