摘要
目的：检测林麝泌香期与发情期血清性激素（睾酮、雌二醇和孕酮）水平的变化,研究其变化规律与泌香的关系。方法：采用磁微粒分离酶联免疫法测定林麝泌香期和发情期血中3种性激素含量。结果：林麝在泌香期3种血清性激素含量变化具有明显规律性,三者同步出现波峰,泌香前期睾酮、雌二醇和孕酮含量均处于较低水平,泌香盛期均升到各自最高峰,泌香末期又均迅速降低到与其泌香前期接近的水平。发情期血清睾酮含量升高,出现峰值较低的波峰,雌二醇维持在较低水平,孕酮含量接近零。泌香盛期的血清睾酮含量是发情期的114.4~190.5倍。在泌香期,3种血清性激素含量的相关性属中等、强正直线相关;产香量与血清睾酮含量呈正相关,与血清雌二醇、孕酮含量和年龄均呈负相关。结论：泌香盛期林麝血清睾酮含量上升到最高,可作为人工诱导麝泌香的参考。
Objective：To detect concentrations of serum gonadal hormones （testosterone, estradiol and progesterone）in musk-secre- ting period and estrus of Moschus berezovskii, and to study the association of serum gonadal hormones concentrations and musk- secreting. Methods ： The concentrations of serum gonadal hormones were detected with magnetic particle separation ELISA. Results ： Dur- ing musk-secreting period,concentration changes of three serum gonadal hormones showed clear regularity, three crests occurred syn- chronously. Before musk-secreting period, testosterone, estradiol and progesterone concentrations were at its lower level, in prime musk- secreting period, they increased rapidly to respective highest peak ; at later musk-secreting period, they quickly dropped to close to its previous levels before musk-secreting period. During estrus, serum testosterone concentration increased to lower peaks than that at later musk-secreting period. Estradiol remained at a low level and progesterone level was closed to zero. Serum testosterone concentrations in prime musk-secreting period were 114. 4 ~ 190. 5 times of estrus. During musk-secreting period, there were significant positive correla- tion among three serum gonadal hormone levels, a positive correlation between musk yield and serum testosterone levels, and negative correlation of musk yield with serum estradiol and progesterone levels as well as musk deer ages. Conclusion：Serum testosterone concen- trations in prime musk-secreting period increase to the highest levels, which can provide reference in musk secretion induced by artifi- cial means.
出处
《中药材》
CAS
CSCD
北大核心
2015年第2期240-244,共5页
Journal of Chinese Medicinal Materials
基金
山西省自然科学基金项目(2012011035-1)
关键词
林麝
血清性激素
泌香期
发情期
睾酮
泌香机理
Moschus berezovskii Flerov
Serum gonadal hormone
Musk-secreting period
Estrus
Testosterone
Musk-secretionmechanism
作者简介
张争明（1962-），男，硕士，教授级高级工程师，研究方向：药用动物资源升发利用；E_mail：deer1980@126．com。
