摘要
目的观察加味二陈汤对Apo E-/-小鼠血脂的影响。方法 44只Apo E-/-小鼠以高脂饲喂养4周构建AS小鼠模型,分别用辛伐他汀、加味二陈汤对小鼠灌胃给药,8周后处死小鼠,酶法检测血清血脂;每周称取并记录小鼠体重;油红O染色观察小鼠主动脉管腔内粥样斑块;HE染色观察小鼠主动脉管腔内粥样斑块沉积及肝细胞形态。结果加味二陈汤可显著降低小鼠血清中TCHOL、LDL-C水平(P<0.01);各组小鼠体重均有所增加,但模型组小鼠体重略高于空白对照组和加味二陈汤组;油红O染色结果显示加味二陈汤可显著减少Apo E-/-小鼠主动脉管腔内粥样斑块的面积;小鼠主动脉HE染色示加味二陈汤组小鼠主动脉内有脂质沉积斑块较模型组明显减少;肝细胞切片HE染色发现加味二陈汤组小鼠肝细胞的病变程度较轻,肝小叶结构较清晰。结论加味二陈汤能有效降低Apo E-/-小鼠血清中有关脂质的含量,调节小鼠脂质代谢,缓解动脉粥样硬化进程。
Objective To explore the effects of the Chinese medicine, modified Erchen decoction, on the serum lipid spectrum of ApoE-/-mice, and to explore its possible anti-atherosclerotic mechanism.Methods Forty-four male 7-8-week old ApoE-/-mice were used in this experiment.ApoE-/-mouse models of atherosclerosis were generated by high-cholesterol diet for 4 weeks.And then, they were given simvastatin or modified Erchen decoction by gavage.The body weight of mice was recorded every week, The mice were sacrificed after treated with the drugs for 8 weeks continuously, and the plasma lipid was determined by enzymatic method.The aortic valves and arches were stained with oil red O to depict atherosclerotic plaques and liver structural changes of the mice were examined by pathology.Results Modified Erchen decoction lowered plasma lipid （ including TCHOL and LDL-C ） significantly （ P〈0.01 ） .The body weight was increased in the mice of all groups, but it was more pronounced in the mice of model group than in the blank and modified Erchen decoction groups.The serum CHOL and LDL-C levels were significantly lowered in the modified Erchen decoction group （P〈0.01）.The area of atherosclerotic plaques in the aortic wall was significantly reduced in the mice of modified Erchen decoction group as shown by oil red O staining.The pathological changes of hepatocytes were less severe and the structure of hepatic lobules was better preserved in the mice of modified Erchen decoction group.Conclusions The Chinese medicin modified Erchen decoction can effectively reduce serum lipids, regulate lipid metabolism, and ameliorate the process of atherosclerosis in ApoE-/-mice.
出处
《中国比较医学杂志》
北大核心
2015年第4期44-47,I0003,共5页
Chinese Journal of Comparative Medicine
基金
全国第五批老中医药专家学术经验继承项目
湖南省教育厅科研青年项目(编号11B092)
2011湖南省大学生研究性学习和创新性实验计划项目(编号481)
湖南省中医药科研计划项目(编号201236)
湖南省中药粉体与创新药物省部共建国家重点实验室培育基地资助
关键词
加味二陈汤
动脉粥样硬化
血脂
HE染色
Modified Erchen decoction
Atherosclerosis
Serum lipid
Aorta
Pathology
ApoE-/-mice
作者简介
[作者简介]谢雪姣，女，医学博士，副教授，硕士生导师，全国第五批老中医药专家学术经验继承人，主要从事心脑血管疾病中医药防治研究与中医人才培养研究，E-mail：99511298@qq．com。
[通讯作者]廖端芳，男，医学博士，教授，博士生导师，主要从事动脉粥样硬化研究，E-mail：dflia066@aliyun．com。
