摘要
利用Design-Expert 7.1.6软件Box-Benhnken中心组合试验和响应面分析法优化苦参中总酚的提取工艺条件。在单因素试验的基础上,考察乙醇浓度、超声时间、液料比和超声温度的单一和交互因素对苦参提取率的影响。结果表明:苦参总酚的最佳提取工艺为乙醇浓度44.20%、超声时间29.50 min、液料比32.50∶1(m L/g)、超声温度57.00℃,在此条件下苦参总酚提取率可达4.51%。
The ultrasonic-assisted extraction process of total phenol from Sophora flavescens was studied using Box-Benhnken center composite design and response surface methodology （RSM）. Based on the experiment of single factor, the impacts of ethanol concentration, extraction time, ratio of liquid to solid, extraction temperature and interaction among them on extraction rate of total phenol was studied by RSM. The result suggested that the optimum extraction conditions were as follow：ethanol concentration of 44.20%, extraction time of 29.50 min ratio of liquid to solid of 32.50∶1（mL/g）, and extraction temperature of 57.00℃. Under these conditions, the practical yield of total phenol reached to 4.51%.
出处
《食品研究与开发》
CAS
北大核心
2015年第5期24-28,共5页
Food Research and Development
基金
山西省高等学校科技创新项目资助(2013149)
忻州师范学院大学生科技创新项目(2013)
关键词
苦参
总酚
响应面分析法
工艺条件
Sophora flavescens
total phenol
response surface methodology
process conditions
作者简介
李海平（1974-），女（汉），实验师，本科，研究方向：天然产物开发。
通信作者：王迎进（1980-），男，讲师，硕士，主要从事天然产物提取、分离及生物活性研究。
