摘要
目的:采用高效液相色谱法测定苦参不同年份、不同部位药材中槐果碱、槐定碱和氧化苦参碱的含量。方法:采用EliteHypersilNH2色谱柱(250mm×4.6mm,5μm),流动相为乙腈-无水乙醇-3%磷酸溶液(82∶10∶8),流速:1mL/min,检测波长:220nm,柱温:26℃,进样量:5μL。结果:槐果碱、槐定碱、氧化苦参碱的线性范围分别为0.00499～0.1497μg(r=0.9999),0.02508～0.75225μg(r=0.9999),0.07538～2.26125μg(r=0.9999);回收率分别为99.91%,99.26%,100.27%,RSD分别为1.11%,0.82%,2.18%。结论:该方法精密度、重现性和分离效果好,苦参不同年份、不同部位中3种生物碱成分含量存在明显差异。研究结果对苦参药材质量评价及苦参全草的有效开发利用具有重要的参考价值。
AIM： To establish an HPLC method for determining sophocarpine,sophoridine and oxymatrine in Sophora flavescens Ait,so as to investigate their contents in different years and different parts. METHODS： An Elite Hypersil NH2 column（250 mm × 4. 6 mm,5 μm） was used with the mobile phase being acetonitrile-absolute alcohol-3% phosphoric acid solution（82 ∶ 10 ∶ 8）,flow rate being 1 mL/min,determinating wavelength being 220 nm,the column temperature being 26 ℃,The injection volume was 5 μL. RESULTS： The calibration curves of sophocarpine,sophoridine and oxymatrine were in good linearity over the ranges of 0. 004 99 - 0. 149 7 μg（r = 0. 999 9）,0. 025 08 - 0. 752 25 μg（r = 0. 999 9）,0. 075 38 - 2. 261 25 μg（r = 0. 999 9）; and the average recovery of sophocarpine,sophoridine and oxymatrine was 99. 91% ,99. 26% ,100. 27% with RSD of 1. 11% , 0. 82% ,2. 18% respectively. CONCLUSION： The HPLC method shows a good separation,reproducibility and accuracy,there are obvious differences in the contents of three alkaloids in different years and different parts of Sophora flavescens Ait. The results provide important data for quality evaluation and utilization of Sophora flavescens materials.
出处
《中成药》
CAS
CSCD
北大核心
2010年第5期790-793,共4页
Chinese Traditional Patent Medicine
基金
贵州省科技厅项目[黔科合SY字(2008)3025
黔科合计省合(2008)7004]
关键词
苦参
槐果碱
槐定碱
氧化苦参碱
含量
Sophora flavescens
sophocarpine
sophoridine
oxymatrine
contents
作者简介
徐石稳（1985-），男，硕士研究生，研究方向：天然药物化学。Tel：（0851）3805459 E-mail：xushiwenl985@163．com
通讯作者：杨小生，男，研究员，博士。Tel：（0851）3805459 E—mail：gzcnp@yahoo．com．cn
