摘要
目的:探讨丁基苯酞预处理对缺血/再灌注大鼠海马迟发性神经元死亡以及热休克蛋白70表达的影响。方法:126只大鼠分为实验对照组(36只)、脑缺血组(36只)、丁基苯酞组(6只)、丁基苯酞+脑缺血组(36只)、槲皮素+丁基苯酞+脑缺血组(6只)、DMSO+丁基苯酞+脑缺血组(6只)。建立大鼠全脑缺血/再灌注模型后。实验对照组、脑缺血组、丁基苯酞+脑缺血组下另加设5个亚组,为手术后6 h、12 h、1 d、3 d、5 d组。用硫堇以及免疫组织化学染色的方法观察丁基苯酞对缺血/再灌注大鼠海马神经元迟发性死亡以及HSP70表达的影响。结果:丁基苯酞预处理可以减轻大鼠全脑缺血/再灌注损伤引起的海马CA1区锥体神经元迟发性死亡,明显增加CA1区HSP70的阳性表达,且持续时间较长(6 h-5 d);应用HSP70抑制剂可以阻断丁基苯酞预处理诱导的大鼠脑缺血耐受。结论:丁基苯酞可能参与脑缺血/再灌注损伤的神经元保护作用,这一作用可能是通过上调HSP70的表达完成的。
Objective： To explore the effect of 3-n-butylphthalide pretreatment on the delayed neuronal death（DND） and the expreesion of heat shock protein70（HSP70） in rat hippocampus after ischemia/repeffusion. Methods： All rats were randomly divided into sham group（ n = 36）, total cerebral ischemia（TCI） group（ n = 36）, butylphthalide（NBP） group（ n = 6）, NBP ＋ TCI group（ n = 36）, quercetin ＋ NBP ＋ TCI group（ n =6）, dimethyl sulfoxide（DMSO） ＋ NBP ＋ TCI group（ n =6）. The model of total cerebral ischemia/reperfusion was estab- lished by blocking vertebral arteries and carotid arteries. In sham group, TCI group and NBP group, the animals were further divided into in- stantly, 6 h, 12 h, 1 d, 3 d, 5 d groups according to the time interval after sham operation or TCI. Histological changes of the hippocmnpus were evaluated using thionin staining under hght microscope by detemaining the delayed neuronal death （DND） and the expression of HSP70 was assayed using immtmohistochemistry. Results： NBP pretreatment could reduce delayed neuronal death in CA1 of hippocampus induced by TCI-reperfusion injury in rats, and up-regulated the expression of HSP70 in CA1 hippocampus of brain ischemic/repeffusion for 5 days. Quercetin blocked the acquirement of the brain ischemic tolerance induced by NBP preconditioning. Conclusion： 3-n-butylphthalide（NBP） prevents the neurons from ischemia/reperfusion injury through upregulating the expression of HSP70.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第2期136-140,I0003,共6页
Chinese Journal of Applied Physiology
关键词
丁基苯酞
脑缺血预处理
HSP70
全脑缺血
大鼠
butylphthalide
brain ischemic preconditioning
HSP70
total cerebral ischemia
rat
作者简介
【通讯作者】Tel：0311—66003912；E-mail：guoli6@163．com
