摘要
目的研究护心康对大鼠动脉粥样硬化防治的作用机制。方法采用高脂喂饲及一次性腹腔注射维生素D3复制大鼠动脉粥样硬化模型,将护心康分为低、中、高3个不同剂量组进行干预治疗,通过免疫组化法检测给药后主动脉中核转录因子-κB(NF-κB)、血管细胞黏附分子-1(VCAM-1)及单核细胞趋化蛋白-1(MCP-1)的表达情况。结果护心康低、中、高剂量组均有较好的降低动脉粥样硬化大鼠主动脉中NF-κB、VCAM-1及MCP-1表达的作用(P<0.01),其中NF-κB、VCAM-1的表达显示出护心康中、高剂量组作用优于低剂量组,差异有统计学意义[NF-κB:(0.236 00±0.015 17)、(0.172 00±0.024 90)比(0.406 00±0.070 21);VCAM-1:(0.462 00±0.085 56)、(0.410 00±0.047 43)比(0.622 00±0.085 56)](P<0.05)。结论护心康具有降低动脉粥样斑块中炎症因子NF-κB、VCAM-1及MCP-1表达的功效,从而抑制大鼠动脉粥样硬化的炎性反应,来达到对动脉粥样硬化的保护作用。
Objective To explore the mechanism of Huxinkang （HXK） on prevention of rats with atherosclerosis （AS）.Methods The rats were fed with high cholesterol diet and given disposable intraperitoneal injection of vitamin D3 to induce the models,then,they were treated by low,middle and high dose of HXK.The levels of nuclear factor gene binding （NF-κB）,vascular cell adhesion molecule-1 （VCAM-1） and monocyte chemoattractant protein-1 （MCP-1） on aortic of AS rats were examined by immunohistochemistry in each group.Results All the low,middle and high dose groups could decrease the expression of aortic NF-κB,VCAM-1 and MCP-1 in rats （P ＜ 0.01）,and the effects in middle and high dose groups were better than the expression of NF-κB,VCAM-1 in low dose group,the differences were statistically significant [NF-κB：（0.236 00±0.015 17）,（0.172 00±0.024 90） vs （0.406 00±0.070 21）;VCAM-1：（0.462 00± 0.085 56）,（0.410 00±0.047 43） vs （0.622 00±0.085 56）] （P ＜ 0.05）.Conclusion HXK can decrease the expression of NF-κB,VCAM-1 and MCP-1 of inflammatory cytokines to inhibit the inflammation in aortic tissue of AS rats,so as to achieve the protective effect on atherosclerosis.
出处
《中国医药导报》
CAS
2015年第13期19-22,F0003,共5页
China Medical Herald
基金
湖南省中医药科研计划项目(60010287)
作者简介
郭乐（1985.9-）,女,硕士;研究方向：中西医内科学.
杨军辉（1969.4-）,男,博士,副教授;研究方向：中医诊断学.
