摘要
脂质转移蛋白是植物生命活动中重要的活性蛋白质,在花粉发育中起着重要作用。本研究以青花菜保持系‘WN12-95B’为试验材料,采用RT-PCR技术获得脂质转移蛋白(LTP)基因c DNA序列。通过生物信息学分析,结果表明:该基因含有一个360 bp的开放阅读框,推导编码119个氨基酸,预测蛋白分子量12.47 k D,p I值7.48,属疏水性蛋白。青花菜LTP蛋白氨基酸序列含有24个氨基酸长度的信号肽,α-螺旋和不规则卷曲是其二级结构的主要成分,三级结构则具有典型的LTP蛋白结构特征。分子进化分析表明该基因与油菜亲缘关系最近,相似性最高,其次为芥菜和芜菁。时空表达特性分析显示,青花菜LTP基因特异表达于花蕾中,且表达丰度较高。保持系和Ogu不育系不同发育阶段花蕾LTP基因的表达丰度差异明显,表达量随花蕾发育呈下降趋势。本研究可为深入研究LTP基因在青花菜绒毡层和小孢子发育过程中的作用提供一定的理论基础。
Lipid transfer proteins are important active proteins in plants, which has a vital function during pollen development. In this study, we used reverse transcript PCR（RT-PCR） technology to get the c DNA sequences of Lipid Transfer Protein（LTP） from a broccoli maintenance line‘WN12-95B＇. Through bioinformatics analysis,the results showed that LTP gene contained a 360 bp open reading frame and encoded 119 amino acid residues. Its relative molecular mass was 12.47 k D, and p I was 7.48. Meanwhile, the lipid transfer protein was a hydrophobic protein. The LTP protein contained a signal peptide of 24 amino acids in length. α-helixs and random coils was the main components of the two-dimension structure of LTP protein. Meanwhile, its three-dimension structure had the typical structure characteristics of LTP. During molecular evolution, the LTP from broccoli had a highest homology with the LTP from Brassica napus（rape）, followed by Brassica juncea and Brassica rapa. The time and space expression characteristic analysis revealed that the express characterisation of LTP gene was tissue-specific and only expressed in bud of broccoli. There were also significant differences in the expressive abundance in buds of different development stage among Ogu male sterile line and its maintenance line, the expression quantity was decreasing with the buds developed. This research can lay the foundation for studying the role of LTP in tapetum and microspore development of broccoli.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第3期615-621,共7页
Molecular Plant Breeding
基金
浙江省自然基金(LY12C15009)
浙江省农业新品种选育重大科技专项(2012C12903-3-3)
浙江省重大科技项目(2010C12004)
浙江省重点科技创新团队项目(2013TD05)共同资助
作者简介
通讯作者，jingzange@aliyun．com
