摘要
为了研究谷胱甘肽S-转移酶基因(GST)家族在甜菜(Beta vulgaris)中对其生长发育及受到非生物胁迫时的潜在功能,以甜菜BvGSTs家族为研究对象,对其理化性质、进化关系、顺式作用元件、染色体定位及在镉胁迫下的转录表达特性进行深入地分析。结果表明:甜菜基因组中共有52个BvGSTs基因家族成员,分布于7个亚家族中;它们的相对分子质量在17~28 kDa,理论等电点pI在5.16~6.77,大部分成员定位于细胞质。BvGSTs结构中共发现9个motif,其中motif 8为Phi亚家族所特有。30个BvGSTs分别位于8条甜菜染色体,存在4处串联重复。根据顺式作用元件分析,甜菜BvGSTs可参与多种生物与非生物胁迫响应。转录组学分析发现全部52个BvGSTs均不同程度的参与到甜菜对镉胁迫的应答过程。其中在地下部,大多数Tau亚家族成员的表达受到镉胁迫的正向调控;在地上部,Phi亚家族受镉胁迫的显著诱导,Tau亚家族的表达被抑制。qRT-PCR分析表明,4个差异表达显著的BvGSTs的转录受到了镉胁迫调控,并与转录组测序结果相符。上述结果为进一步对甜菜谷胱甘肽S-转移酶在镉胁迫过程中的生物学功能的研究奠定基础。
To clarify the potential function of Glutathione-S-transferase gene(GST)family in sugar beet(Beta vulgaris)growth and development and abiotic stress,the sugar beet Bv GSTs was identified,and the physical and chemical properties,evolutionary relationship,homeopathic elements,chromosome location and transcriptional expression characteristics under cadmium stress were comprehensively analyzed,respectively.The results showed that there were 52 members of Bv GSTs in sugar beet genome,which were distributed in seven subfamilies;The molecular weight of the members ranged from 17-28 k Da,and the theoretical isoelectric point(p I)was between 5.16-6.77,and most of the members were located in cytoplasm.There were nine motifs found in Bv GSTs,among which motif eight was unique to Phi subfamily.Among all the members,30Bv GSTs were distributed in eight chromosomes respectively,with four tandem repeats.According to cis-acting element analysis,Bv GSTs might be involved in a variety of biotic and abiotic stress responses.Transcriptome analysis showed that all of 52 Bv GSTs were related to response of sugar beet to cadmium stress.The expression of most members of Tau subfamily under ground parts was positively regulated by cadmium stress;Phi subfamily above ground parts was significantly induced by cadmium stress,and the expression of Tau family was inhibited.The result of q RT-PCR showed that the transcripts of the four differentially expressed Bv GSTs were regulated by cadmium stress,which was consistent with the results of transcriptome sequencing.These results would play a foundamental role in further study on the biological function of sugar beet glutathione S-transferase under cadmium stress.
作者
钟鑫爱
孟诗琪
周婉婷
姚琦
张琼
兴旺
刘大丽
ZHONG Xin’ai;MENG Shiqi;Zhou Wanting;YAO Qi;ZHANG Qiong;XING Wang;LIU Dali(National Beet Medium-term Gene Bank,Heilongjiang University,Harbin 150080;Key Laboratory of Sugar Beet Genetics and Breeding,College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080;Key Laboratory of Molecular Biology,School of Life Sciences,Heilongjiang University,Harbin 150080)
出处
《植物研究》
CAS
CSCD
北大核心
2022年第5期790-801,共12页
Bulletin of Botanical Research
基金
国家作物种质资源库“甜菜分库运行服务”(NCGRC-2021-017)
农业农村部“甜菜种质资源的收集、鉴定、编目、繁种与入库(圃)保存”(19210157)
普查收集甜菜种质资源鉴定评价与编目入库(19210911)
甜菜种质资源安全保存(19211031)
国家糖料产业技术体系项目(甜菜种质资源鉴定与新种质创制)(CARS-170102)
黑龙江省自然科学基金项目(LH2019C057)
黑龙江省高校基本科研业务费黑龙江大学专项资金项目(KJCX201920)。
关键词
甜菜
全基因组鉴定
谷胱甘肽S-转移酶
镉胁迫
sugar beet
genome-wide identification
glutathione S-transferase(GST)
cadmium stress
作者简介
第一作者:钟鑫爱(1998-),女,硕士研究生,主要从事作物分子生物学方面研究;通信作者:刘大丽,E-mail:daliliu_hlju@163.com。
