摘要
目的:检测慢性乙型肝炎患者外周血中Treg细胞的比例,探讨其对DC细胞的免疫抑制作用。方法:检测CHB患者和正常对照组外周血CD4+,CD25+,Treg及DC表面因子CD80和HLA-DR的表达;在DC培养不同时间内加入不同比例CHB Treg细胞,观察DC对淋巴细胞增殖的影响以及细胞因子IL-10及TGF-β的表达变化。结果:CHB患者外周血Treg细胞比例高于对照组,差异有统计学意义(P<0.05);CHB患者DC细胞表面分子CD80和HLA-DR表达均低于对照组,差异有统计学意义(P<0.05);经Treg细胞处理后,DC细胞刺激淋巴细胞的增殖能力下降,淋巴细胞增殖的抑制率显著升高(P<0.05);随Treg细胞增加,IL-10及TGF-β水平升高,差异有统计学意义(P<0.05)。结论:CHB患者外周血中Treg细胞的增加可能通过诱导IL-10和TGF-β的表达来抑制DC细胞的免疫功能。
Objective： To investigate the Treg cells suppressing the dendritic cells in the pathogenesis of chronic hepatitis B.Methods： CD4＋CD25＋regulatory T cells and the expression of CD80 and HLA-DR on DC in patients with CHB and the control group were detected. Treg cells of CHB and DC of the controls were cultured with different ratio and time, then the concentrations of IL-10 and TGF- β were detected by ELISA. Results： The frequency of Treg cells in CHB group was higher than that of the control group with statistically significant difference（P0.05）; The expressions of CD80 and HLA-DR in CHB group were lower than those of the control group with statistically significant difference（P0.05）; Then CD4＋CD25＋regulatory T cells in CHB group could significantly suppress effects on DC immunity with time-and-dose depended; The secretion of IL-10 and TGF-β increased significant in CHB group（P0.05）.Conclusions： The increasing Treg cells of patients with CHB may suppress DC by inducing the expressions of IL-10 and TGF-β.
出处
《现代生物医学进展》
CAS
2015年第11期2053-2055,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金青年科学基金项目(30901795)
作者简介
余灵祥（1976-），主治医师，主要研究方向：肝病外科诊断与手术治疗
通讯作者：张绍庚，医学博士，教授，主任医师，主要研究方向：肝脏肿瘤、胆道外科、门脉高压症和腹腔镜微创外科手术等，E—mail：laohushanshang@163．com
