摘要
香蕉与枯萎病互作机理研究一直是近年来研究的热点,目前对枯萎病菌的致病机理及香蕉的感、抗病机理还不清楚。本文利用前期表达谱分析结果克隆了乙烯合成及调控途径中的关键酶基因乙烯形成酶基因(MaACO)和乙烯响应因子基因(MaERF1),通过RT-PCR检测,对这两个基因在感、抗病香蕉种质中的表达水平进行研究。结果表明,MaACO和MaERF1基因对机械损伤非常敏感,尤其在损伤初期(3h)的表达水平远高于后期;对于枯萎病菌侵染处理的植株,尤其在感染初期(3h)MaACO和MaERF1的表达水平在抗性植株中的表达量均比感病植株中的低;抗性种质中乙烯途径可能受到抑制,香蕉的感病性可能与侵染初期对乙烯信号的敏感性相关。本研究为利用乙烯抑制剂进行香蕉抗枯萎病新方法的研究奠定了基础。
The banana wilt caused by Fusarium oxysporum f. sp. c ubense （Foc 4） has brought destructive attack for banana planting. More research has focused on the pathogen, pathogenesis and disease-resistance mechanism of ba- nana in recent years. To explore the response pattern between Foc 4 and banana at early stage, two important genes involved in ethylene biosynthesis and regulation from banana, i.e. 1 aminocyclopropane 1 carboxylate oxidase （MaA CO） and ethylene responsive transcription factor 1 （MaERF1）, were investigated in detail based on RT-PCR. It was found that all genes were more sensitive to the damage at 3 h than at 27 h and 51 h, respectively, for all banana plants. Furthermore, it was noted that the expression level of MaA CO and MaERF1 was lower in disease-resistant ba- nana than the disease-sensitive banana especially at the early stage （3 h）, suggesting ethylene signal transduction might be inhibited in these disease-resistant banana. We reckoned that ethylene biosynthesis might be negatively re- lated with the resistance of banana to Foc 4 at least for the early stage of infection. More research needs to be done to explore the relationship between banana resistance and ethylene signal transduction in the future.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2014年第5期1013-1018,共6页
Genomics and Applied Biology
基金
中国热带农业科学院院本级基本科研业务费专项(1630052014001)
海南省重大科技项目子课题(ZDZX2013023-1)
海南省自然基金(311078)共同资助
关键词
香蕉
枯萎病菌
MAA
CO
MaERF1
表达水平
Banana, Fusarium oxysporum f. sp. cubense （Foc）, MaA CO, MaERF1, Expression level
作者简介
通讯作者，mmpeng_2000@yahoo．com
