摘要
【目的】对广东省中山市某养殖场的患病濒死杂交鳢进行病原分离鉴定及药敏试验,旨在确定病因,为今后生产中有效防控该病提供参考。【方法】从濒死杂交鳢体内分离致病菌,通过其生理生化特征与16S r RNA基因序列分析进行菌株鉴定,并采用纸片扩散法进行药敏试验。【结果】从患病濒死的杂交鳢体内分离获得两株优势菌(ZS2013711-1和ZS2013711-2),根据其生理生化鉴定结果与16S r RNA基因序列分析结果,可鉴定为海豚链球菌(Streptococcus iniae)。两株分离菌株对杂交鳢的半致死剂量(LD50)分别为2.7×105和6.7×105 CFU/g。药敏试验结果显示,该致病菌对头孢噻肟、头孢拉定、头孢唑啉、林可霉素等抗生素高度敏感。【结论】杂交鳢是海豚链球菌的一种新发现宿主,生产中可选用头孢噻肟、头孢拉定、头孢唑啉、林可霉素等药物进行防治。
【Objective 】The pathogens from diseased hybrid snakehead（Channa argus♂×Channa maculata♀） in an aquafarm in Zhongshan City of Guangdong Province were isolated and identified and antibiotic sensitivity test was performed in order to determine the causes and provide references for preventing and controlling this disease effectively.【Method】Pathogenic bacteria were isolated from moribund hybrid snakehead, and they were identified through physiological and biochemical characteristics test and 16 S r RNA sequence analysis. Furthermore, antibiotic sensitivity test was performed through Kirby-Bauer disk diffusion method. 【Result】Two dominant bacterias（ZS2013711-1, ZS2013711-2）were isolated from moribund hybrid snakehead, and they were identified to be Streptococcus iniae according to their physiological and biochemical characteristics and 16 S r RNA sequence analysis. The half lethal dose values for the isolates to healthy hybrid snakehead were 2.7 ×105and 6.7 ×105CFU/g, respectively. The results of antibiotic sensitivity test showed the isolates were highly sensitive to cefotaxime, cefradine, cephazolin, lincomycin, and so on. 【Conclusion】Hybrid snakehead is a new found host for S. iniae. In hybrid snakehead aquaculture industry, its disease resulted from S. iniae can be prevented and treated by cefotaxime, cefradine, cephazolin and lincomycin.
出处
《南方农业学报》
CAS
CSCD
北大核心
2015年第2期332-337,共6页
Journal of Southern Agriculture
基金
广东省教育部产学研合作项目(2010b090400255)
广东省普通高校青年创新人才项目(2014KQNCX183)
佛山科学技术学院科研项目(2014)
关键词
杂交鳢
海豚链球菌
分离
鉴定
药敏试验
hybrid snakehead
S.iniae
isolation
identification
antibiotic sensitivity test
作者简介
陈言峰（1981-），博士，主要从事鱼类病害研究工作，E-mail：chyfwf@126．com
为通讯作者，张辉华（1971-），教授，主要从事鱼类生态养殖技术研究工作，E—mail：hhzhang2@163.com。
