摘要
为了探讨牡丹在衰老过程中叶片光合机构活性的变化与光合能力的维持关系,本试验以早衰品种‘肉芙蓉’和晚衰品种‘卷叶红’为材料,通过快速叶绿素荧光诱导动力学测定技术(JIP test)和820nm光吸收技术,研究牡丹在衰老过程中的净光合速率(Pn)、叶绿素含量及光合机构活性的变化。结果表明:在衰老进程中,牡丹Pn和叶绿素含量以相同的趋势下降,肉芙蓉’快于‘卷叶红’的下降。通过对荧光参数的分析,发现牡丹叶在衰老时,PSⅡ电子受体侧受到的伤害大于电子供体测,而单位面积有活性的反应中心数量(RC/CSo)显著下降,同时PSⅠ的活性也大幅下降。无论PSⅡ还是PSⅠ的活性,肉芙蓉’的下降幅度都大于‘卷叶红’。由此得出:PSⅠ和PSⅡ光系统活性的快速下降和光合电子传递能力的衰退是牡丹叶片光合能力迅速下降的重要原因之一。
In order to investigate the connection between changes of potosystem activity and photosynthetic capacity of tree peony leave during the aging process,premature senescenc Roufurong and late period senescence Juanyehong were used as material in this experiment and the net photosynthetic rate（Pn）,chlorophyll content and photosynthetic activity were studied through analyzing chlorophyll a fluorescence transient and 820 nm transmission.The results show that：the Pnand chlorophyll content decreased in the same trend in the aging process of tree peony,but Roufurong decreased faster than Juanyehong.It was also found that the damage in electron acceptor side of PS Ⅱ was greater than the electron donor.The density of RCs per CS（RC/CSo）was significantly decreased by analyzing the fluorescence parameters.PSⅠ activity also decreased significantly at the same time.PSⅡ and PSⅠactivity of Roufurong declined more than that of Juanyehong.So we draw a conclusion：the faster decline of potosystem activities of PSⅠ and PSⅡand severer deterioration of electron transfer activity are two of the most important reasons to cause earlier senescence in premature senescence of tree peony.
出处
《青岛农业大学学报（自然科学版）》
2015年第1期27-30,41,共5页
Journal of Qingdao Agricultural University(Natural Science)
基金
青岛农业大学高层次人才启动基金资助(631413)
关键词
牡丹
衰老
光系统活性
叶绿素荧光测定技术
tree peony
senescence
photosystem activity
chlorophyll a fluorescence transient
作者简介
杨德翠（1974-），女，山东曹县人，博士，讲师，主要从事植物逆境生理与分子生物学研究
