摘要
目的对打破碗花花根茎的化学成分进行研究。方法样品用70%的乙醇进行提取,所得浸膏用水分散后,经石油醚-正丁醇系统进行萃取,将所得正丁醇层经正相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、ODS反相柱色谱及HPLC等分离手段分离得到单体化合物,根据其波谱学数据及理化性质鉴定其化学结构。结果分离得到5个单体化合物,分别被鉴定为cauloside D(1)、huzhangoside B(2)、huzhangoside C(3)、hederacolchiside E(4)、clematiganoside A(5)。结论除huzhangoside B外,其余4个化合物均为首次从该植物中分离得到。
Objective To determine the chemical constituents from the rhizome of Anemone hupehensis Lem.. Methods Rhizome of Anemone hupehensis Lem. was extracted with 70% Et OH, and the residue was suspended in H2 O and extracted with petroleum ether and n-butanol, respectively. Column chromatographies（including silica gel, Sephadex LH-20 and ODS） and preparative HPLC were used to separate the n-butanol section. The structures were elucidated on the basis of spectroscopic data and chemical derivatization method. Results Five compounds were separated and identified as cauloside D（1）, huzhangoside B（2）, huzhangoside C（3）, hederacolchiside E（4）, and clematiganoside A（5）. Conclusion All the compounds except for huzhangoside B have been isolated from Amenone hupehensis for the first time.
出处
《中南药学》
CAS
2015年第2期128-132,共5页
Central South Pharmacy
关键词
打破碗花花
三萜皂苷
化学成分
Anemone hupehensis Lem.
triterpenoid saponin
chemical composition
作者简介
王夏茵，女，硕士研究生，主要从事天然药化研究，Tel：15091754709，E-mail：xiayinlove@163．com
通讯作者：汤海峰，男，博士研究生导师，主要从事天然药化研究和中药、海洋药物开发，Tel：（029）84774748，E-mail：tanghaifeng71@163．com；
通讯作者：涂宏海，女，主任药师，主要从事生药学研究，Tel：13319802858，E-mail：tuhhai@263．net
