摘要
目的分析微粒色谱法和高效液相色谱法在糖化血红蛋白(HbA1c)检测中的相关性和临床符合情况。方法依照美国临床和实验室标准协会(CLSI)的EP9-A2文件要求,每日随机抽取重庆市急救中心门诊或住院患者血液标本8份,连续5d,共采取40份。分别用微粒色谱法和高效液相色谱法测定HbA1c,并对数据进行比较分析。结果对两种方法测定的HbA1c结果进行比对分析,结果表明两检测系统内重复性好,符合相关性实验要求;方法间无离群点存在;线性回归方程为Y=1.088 5 X-0.521 4,相关系数r=0.993,95%置信区间(B^C,low,B^C,high)为(0.019 1,0.181 8),在允许误差(-0.35,0.35)之内,两种方法测定结果无明显差异,不存在有意义的偏倚。结论微粒色谱法和高效液相色谱法可比性高,相关性好,符合临床需求,检验科可根据自身条件,选择适合的测定方法。
Objective To analyze the relationship between the particulate chromatography and the high performance liquid chromatography（HPLC）in detecting glycosylated hemoglobin（HbA1c）and their clinical conformance situation.Methods According to the Clinical and Laboratory Standard Institute（CLSI）EP9-A2 document,8blood samples per day were randomly extracted for 5consecutive days,a total of 40 samples were collected.The HbA1 cin each sample was detected by the particle chromatography and HPLC respectively,and the detection data were performed the comparative analysis.Results The comparative analysis showed that the two systems had was repeatability and could meet the criteria of the related laboratory tests.The outlier values were not found.The equation of linear regression was Y=1.088 5 X-0.521 4,the correlation coefficient r=0.993,95% confidence interval：（B^C,low,B^C,high）was（0.019 1,0.181 8）,which was less than the allowable error（-0.35,0.35）.There were no significant differences in the determination results of HbA1 cbetween the two methods.No meaningful bias was found.Conclusion The particulate chromatography and HPLC could have high comparability and good correlation,and could meet the clinical demand.The clinical laboratory could choose the appropriate method according to its own conditions.
出处
《检验医学与临床》
CAS
2015年第5期641-643,共3页
Laboratory Medicine and Clinic
关键词
糖化血红蛋白
相关性
微粒色谱法
高效液相色谱法
glycosylated hemoglobin
correlation
particulate chromatography
high performance liquid chromatography
作者简介
杨朝美，女，本科，主管检验技师，主要从事生化检验研究。
通讯作者，E—mail：mzhen100@163．com。
